DBH Protein (AA 40-617) (His tag)
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- Target See all DBH Proteins
- DBH (Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 40-617
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This DBH protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MSAPRESPLP YHIPLDPEGS LELSWNVSYT QEAIHFQLLV RRLKAGVLFG MSDRGELENA DLVVLWTDGD TAYFADAWSD QKGQIHLDPQ QDYQLLQVQR TPEGLTLLFK RPFGTCDPKD YLIEDGTVHL VYGILEEPFR SLEAINGSGL QMGLQRVQLL KPNIPEPELP SDACTMEVQA PNIQIPSQET TYWCYIKELP KGFSRHHIIK YEPIVTKGNE ALVHHMEVFQ CAPEMDSVPH FSGPCDSKMK PDRLNYCRHV LAAWALGAKA FYYPEEAGLA FGGPGSSRYL RLEVHYHNPL VIEGRNDSSG IRLYYTAKLR RFNAGIMELG LVYTPVMAIP PRETAFILTG YCTDKCTQLA LPPSGIHIFA SQLHTHLTGR KVVTVLVRDG REWEIVNQDN HYSPHFQEIR MLKKVVSVHP GDVLITSCTY NTEDRELATV GGFGILEEMC VNYVHYYPQT QLELCKSAVD AGFLQKYFHL INRFNNEDVC TCPQASVSQQ FTSVPWNSFN RDVLKALYSF APISMHCNKS SAVRFQGEWN LQPLPKVIST LEEPTPQCPT SQGRSPAGPT VVSIGGGKG
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product DBH Protein
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Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH) (AA 494-603) protein (GST tag)
DBH Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH) (AA 40-617) protein (His tag)DBH Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH) (AA 38-617) protein (His tag)Crystallography grade DBH Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH) (AA 42-621) protein (His tag)Crystallography grade DBH Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH) (AA 1-617) protein (Strep Tag)Crystallography grade DBH Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH) (AA 40-617) protein (rho-1D4 tag)Crystallography grade DBH Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH) (AA 44-622) protein (rho-1D4 tag)Crystallography grade DBH Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH) (AA 1-622) protein (Strep Tag)Crystallography grade DBH Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid, In Phosphate buffered saline ( pH 7.4) containing 10 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- DBH (Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH))
- Alternative Name
- DBH (DBH Products)
- Synonyms
- CG1543 Protein, DBH Protein, Dbh Protein, Dmel\\CG1543 Protein, T beta h Protein, TBH Protein, TBetaH Protein, TBetah Protein, TBh Protein, TbetaH Protein, TbetaM Protein, Tbetah Protein, tbh Protein, DBM Protein, DOPBHY Protein, Tbh Protein, dopamine beta-hydroxylase (dopamine beta-monooxygenase) Protein, Tyramine beta hydroxylase Protein, MOXD1 homolog 2 Protein, dopamine beta hydroxylase Protein, dopamine beta-hydroxylase Protein, dbh Protein, Tbh Protein, LOC5574699 Protein, CpipJ_CPIJ004736 Protein, CpipJ_CPIJ004737 Protein, CpipJ_CPIJ014202 Protein, CpipJ_CPIJ016745 Protein, CpipJ_CPIJ019034 Protein, DBH Protein, Dbh Protein, LOC101110668 Protein
- Background
- DBH also known as dopamine beta hydroxylase that catalyzes the chemical reaction. DBH belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with reduced ascorbate as one donor, and incorporation of one ato of oxygen into the other donor. Recombinant human DBH, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 67.2kDa (599aa)
- NCBI Accession
- NP_000778
- UniProt
- P09172
- Pathways
- Carbohydrate Homeostasis
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