NUBP1 Protein (AA 1-320) (His tag)
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- Target See all NUBP1 Proteins
- NUBP1 (Nucleotide Binding Protein 1 (NUBP1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-320
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NUBP1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMEEVPHD CPGADSAQAG RGASCQGCPN QRLCASGAGA TPDTAIEEIK EKMKTVKHKI LVLSGKGGVG KSTFSAHLAH GLAEDENTQI ALLDIDICGP SIPKIMGLEG EQVHQSGSGW SPVYVEDNLG VMSVGFLLSS PDDAVIWRGP KKNGMIKQFL RDVDWGEVDY LIVDTPPGTS DEHLSVVRYL ATAHIDGAVI ITTPQEVSLQ DVRKEINFCR KVKLPIIGVV ENMSGFICPK CKKESQIFPP TTGGAELMCQ DLEVPLLGRV PLDPLIGKNC DKGQSFFIDA PDSPATLAYR SIIQRIQEFC NLHQSKEENL ISS
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product NUBP1 Protein
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Nucleotide Binding Protein 1 (NUBP1) (AA 1-320) protein (His tag)
NUBP1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Nucleotide Binding Protein 1 (NUBP1) (AA 1-320) protein (GST tag)NUBP1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Nucleotide Binding Protein 1 (NUBP1) (AA 222-320) protein (GST tag)NUBP1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Nucleotide Binding Protein 1 (NUBP1) (AA 1-320) protein (Strep Tag)Crystallography grade NUBP1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Nucleotide Binding Protein 1 (NUBP1) (AA 1-320) protein (Strep Tag)Crystallography grade NUBP1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid, In Phosphate buffered saline ( pH 7.4) containing 10 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- NUBP1 (Nucleotide Binding Protein 1 (NUBP1))
- Alternative Name
- NuBP1 (NUBP1 Products)
- Synonyms
- DDBDRAFT_0169230 Protein, DDBDRAFT_0232424 Protein, DDB_0169230 Protein, DDB_0232424 Protein, NBP Protein, NBP1 Protein, nbp Protein, nbp1 Protein, nubp1 Protein, NBP 1 Protein, im:7144207 Protein, zgc:92138 Protein, nucleotide binding protein 1 Protein, nucleotide binding protein 1 (MinD homolog, E. coli) Protein, nucleotide binding protein 1 L homeolog Protein, nucleotide binding protein 1 S homeolog Protein, Nubp1 Protein, nubp1 Protein, NUBP1 Protein, nubp1.L Protein, nubp1.S Protein
- Background
- NuBP1 also known as cytosolic Fe-S cluster assembly factor NuBP1 isoform 1. NuBP1 is implicated in the regulation of centrosome duplication by similarity. This protein is component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. It required for maturation of extra mitochondrial Fe-S proteins The NuBP1-NuBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of a Fe-S cluster and its transfer to target apoproteins. Recombinant human NuBP1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 36.9kDa (343aa) confirmed by MALDI-TOF.
- NCBI Accession
- NP_002475
- UniProt
- P53384
- Pathways
- Transition Metal Ion Homeostasis
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