PI3 Protein (AA 23-117) (His tag)
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- Target See all PI3 Proteins
- PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 23-117
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This PI3 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- AVTGVPVKGQ DTVKGRVPFN GQDPVKGQVS VKGQDKVKAQ EPVKGPVSTK PGSCPIILIR CAMLNPPNRC LKDTDCPGIK KCCEGSCGMA CFVPQHHHHH H
- Purity
- > 85 % by SDS - PAGE.
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Top Product
- Discover our top product PI3 Protein
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Peptidase Inhibitor 3, Skin-Derived (PI3) (AA 1-117) protein (GST tag)
PI3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Peptidase Inhibitor 3, Skin-Derived (PI3) (AA 61-117) protein (His-DYKDDDDK-Strep II Tag)PI3 Origin: Human Host: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
Peptidase Inhibitor 3, Skin-Derived (PI3) (AA 61-117) protein (His tag)Crystallography grade PI3 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Peptidase Inhibitor 3, Skin-Derived (PI3) (AA 61-117) protein (His-SUMO Tag)PI3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Peptidase Inhibitor 3, Skin-Derived (PI3) (AA 23-117) protein (His tag)PI3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 80 % by SDS - PAGE SDS
Peptidase Inhibitor 3, Skin-Derived (PI3) (AA 61-117) protein (His tag)PI3 Origin: Human Host: Yeast Recombinant > 90 % SDS
Peptidase Inhibitor 3, Skin-Derived (PI3) protein (Myc-DYKDDDDK Tag)PI3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 mg/mL
- Buffer
- Liquid. In phosphate buffered saline ( pH 7.4), 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))
- Alternative Name
- PI3 (PI3 Products)
- Synonyms
- ESI Protein, SKALP Protein, WAP3 Protein, WFDC14 Protein, cementoin Protein, trappin-2 Protein, TRAPPIN-2 Protein, elafin Protein, peptidase inhibitor 3 Protein, peptidase inhibitor 3, skin-derived (SKALP) Protein, PI3 Protein
- Background
- PI3, also known as elafin or peptidase inhibitor 3, is neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis. Recombinant human PI3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 10.7kDa (101aa) confirmed by MALDI-TOF. 8.5-13.5kDa (SDS-PAGE under reducing conditions.)
- NCBI Accession
- NP_002629
- UniProt
- P19957
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