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CST5 Protein (AA 26-142) (His tag)

CST5 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90 % by SDS - PAGE SDS Active
Catalog No. ABIN5854281
  • Target See all CST5 Proteins
    CST5 (Cystatin D (CST5))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Protein Characteristics
    AA 26-142
    Origin
    Human
    Source
    • 2
    • 2
    • 2
    • 1
    • 1
    Baculovirus infected Insect Cells
    Purification tag / Conjugate
    This CST5 protein is labelled with His tag.
    Application
    SDS-PAGE (SDS)
    Sequence
    QSRTLAGGIH ATDLNDKSVQ RALDFAISEY NKVINKDEYY SRPLQVMAAY QQIVGGVNYY FNVKFGRTTC TKSQPNLDNC PFNDQPKLKE EEFCSFQINE VPWEDKISIL NYKCRKVHHH HHH
    Purity
    > 90 % by SDS - PAGE
    Endotoxin Level
    < 1.0 EU per 1 microgram of protein (determined by LAL method)
    Biological Activity Comment
    The IC50 value is < 2.0nM. The inhibitory function of Cystatin 5 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.
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  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Bioactivity Validated

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Liquid. In phosphate buffered saline (  pH 7.4) containing 10 % glycerol.
    Storage
    4 °C,-20 °C,-80 °C
    Storage Comment
    Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
  • Target
    CST5 (Cystatin D (CST5))
    Alternative Name
    CST5 (CST5 Products)
    Synonyms
    Cst10 Protein, Cystatin-5 Protein, cystatin D Protein, CST5 Protein, Cst5 Protein
    Background
    CST5, also known as Cystatin-D, is a novel member of the cystatin superfamily. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. Two alleles of the cystatin D gene (CST5), encoding protein variants with either Cys or Arg as residue 26 in their 122-residue polypeptide chains, are present in the population. Recombinant human CST5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
    Molecular Weight
    14.36kDa (123aa) (13.5-18kDa in SDS-PAGE under reducing conditions.)
    NCBI Accession
    NP_001891
    UniProt
    P28325
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