CA12 Protein (AA 25-301) (His tag)
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- Target See all CA12 Proteins
- CA12 (Carbonic Anhydrase 12 (CA12))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 25-301
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This CA12 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- APVNGSKWTY FGPDGENSWS KKYPSCGGLL QSPIDLHSDI LQYDASLTPL EFQGYNLSAN KQFLLTNNGH SVKLNLPSDM HIQGLQSRYS ATQLHLHWGN PNDPHGSEHT VSGQHFAAEL HIVHYNSDLY PDASTASNKS EGLAVLAVLI EMGSFNPSYD KIFSHLQHVK YKGQEAFVPG FNIEELLPER TAEYYRYRGS LTTPPCNPTV LWTVFRNPVQ ISQEQLLALE TALYCTHMDD PSPREMINNF RQVQKFDERL VYTSFSQVQV CTAAGLSHHH HHH
- Purity
- > 90 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 300 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.
- Top Product
- Discover our top product CA12 Protein
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Carbonic Anhydrase 12 (CA12) (AA 25-301) protein (His tag)
CA12 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Carbonic Anhydrase 12 (CA12) (AA 25-299) protein (His tag)CA12 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Carbonic Anhydrase 12 (CA12) (AA 1-354) protein (GST tag)CA12 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Carbonic Anhydrase 12 (CA12) (AA 25-124) protein (GST tag)CA12 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Carbonic Anhydrase 12 (CA12) protein (His tag)CA12 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Carbonic Anhydrase 12 (CA12) (Active) protein (His tag)CA12 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
Carbonic Anhydrase 12 (CA12) (AA 25-301) protein (His tag)Crystallography grade CA12 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Carbonic Anhydrase 12 (CA12) (AA 25-301) protein (His tag)Crystallography grade CA12 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Carbonic Anhydrase 12 (CA12) (AA 25-354) protein (rho-1D4 tag)Crystallography grade CA12 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Carbonic Anhydrase 12 (CA12) (AA 25-354) protein (rho-1D4 tag)Crystallography grade CA12 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In phosphate buffered saline ( pH 7.4) containing 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- CA12 (Carbonic Anhydrase 12 (CA12))
- Alternative Name
- CA12 (CA12 Products)
- Synonyms
- CAXII Protein, HsT18816 Protein, CAR12 Protein, 2310047E01Rik Protein, AI314958 Protein, Ca12 Protein, ca12 Protein, carbonic anhydrase 12 Protein, carbonic anhydrase XII L homeolog Protein, CA12 Protein, Car12 Protein, ca12.L Protein
- Background
- CA12, also known as Carbonic anhydrase 12, is an enzyme that in humans is encoded by the CA12 gene. Carbonic anhydrases(CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10 % of clear cell renal carcinomas. Recombinant human CA12, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 31.94kDa (283aa) (28-40kDa in SDS-PAGE under reducing conditions.)
- NCBI Accession
- NP_001209
- UniProt
- O43570
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