ACP5 Protein (AA 22-325) (His tag)
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- Target See all ACP5 Proteins
- ACP5 (Acid Phosphatase 5, Tartrate Resistant (ACP5))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 22-325
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This ACP5 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- ATPALRFVAV GDWGGVPNAP FHTAREMANA KEIARTVQIL GADFILSLGD NFYFTGVQDI NDKRFQETFE DVFSDRSLRK VPWYVLAGNH DHLGNVSAQI AYSKISKRWN FPSPFYRLHF KIPQTNVSVA IFMLDTVTLC GNSDDFLSQQ PERPRDVKLA RTQLSWLKKQ LAAAREDYVL VAGHYPVWSI AEHGPTHCLV KQLRPLLATY GVTAYLCGHD HNLQYLQDEN GVGYVLSGAG NFMDPSKRHQ RKVPNGYLRF HYGTEDSLGG FAYVEISSKE MTVTYIEASG KSLFKTRLPR RARPHHHHHH
- Purity
- > 95 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 5,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmoles of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C.
- Top Product
- Discover our top product ACP5 Protein
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Acid Phosphatase 5, Tartrate Resistant (ACP5) (Active) protein (His tag)
ACP5 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
Acid Phosphatase 5, Tartrate Resistant (ACP5) (AA 1-325) protein (GST tag)ACP5 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Acid Phosphatase 5, Tartrate Resistant (ACP5) (AA 221-325) protein (GST tag)ACP5 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Acid Phosphatase 5, Tartrate Resistant (ACP5) (AA 72-158) protein (GST tag)ACP5 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Acid Phosphatase 5, Tartrate Resistant (ACP5) (AA 22-325) protein (His tag)Crystallography grade ACP5 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Acid Phosphatase 5, Tartrate Resistant (ACP5) (AA 23-327) protein (His tag)Crystallography grade ACP5 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- ACP5 (Acid Phosphatase 5, Tartrate Resistant (ACP5))
- Alternative Name
- ACP5 (ACP5 Products)
- Synonyms
- SPENCDI Protein, TRAP Protein, acp5 Protein, sb:cb576 Protein, zgc:63825 Protein, wu:fb19f01 Protein, wu:fb30b03 Protein, wu:fi14e01 Protein, wu:fj66f03 Protein, MGC78938 Protein, MGC89674 Protein, TR-AP Protein, zgc:92339 Protein, TRACP Protein, TTRRAP Protein, Trap Protein, UF Protein, acid phosphatase 5, tartrate resistant Protein, acid phosphatase 5a, tartrate resistant Protein, acid phosphatase 5, tartrate resistant S homeolog Protein, tartrate resistant acid phosphatase Protein, Tartrate-resistant acid phosphatase type 5 Protein, tartrate-resistant acid phosphatase type 5 Protein, acid phosphatase 5b, tartrate resistant Protein, ACP5 Protein, acp5a Protein, acp5.S Protein, acp5 Protein, NAEGRDRAFT_81291 Protein, ppa5 Protein, LOC100282899 Protein, acp5b Protein, Acp5 Protein
- Background
- ACP5, also known as tartrate-resistant acid phosphatase type 5, is involved in osteopontin and bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias. Recombinant human ACP5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 35.1kDa (310aa) 28-40kDa (SDS-PAGE under reducing conditions)
- NCBI Accession
- NP_001104505
- UniProt
- P13686
- Pathways
- Transition Metal Ion Homeostasis
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