AKR1D1 Protein (AA 1-326)
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- Target See all AKR1D1 Proteins
- AKR1D1 (Aldo-Keto Reductase Family 1, Member D1 (AKR1D1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-326
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Sequence
- MDLSAASHRI PLSDGNSIPI IGLGTYSEPK STPKGACATS VKVAIDTGYR HIDGAYIYQN EHEVGEAIRE KIAEGKVRRE DIFYCGKLWA TNHVPEMVRP TLERTLRVLQ LDYVDLYIIE VPMAFKPGDE IYPRDENGKW LYHKSNLCAT WEAMEACKDA GLVKSLGVSN FNRRQLELIL NKPGLKHKPV SNQVECHPYF TQPKLLKFCQ QHDIVITAYS PLGTSRNPIW VNVSSPPLLK DALLNSLGKR YNKTAAQIVL RFNIQRGVVV IPKSFNLERI KENFQIFDFS LTEEEMKDIE ALNKNVRFVE LLMWRDHPEY PFHDEY
- Purity
- > 95 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Top Product
- Discover our top product AKR1D1 Protein
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Aldo-Keto Reductase Family 1, Member D1 (AKR1D1) (AA 1-326) protein (GST tag)
AKR1D1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Aldo-Keto Reductase Family 1, Member D1 (AKR1D1) (AA 1-326) protein (His tag)AKR1D1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Aldo-Keto Reductase Family 1, Member D1 (AKR1D1) (AA 227-326) protein (GST tag)AKR1D1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Aldo-Keto Reductase Family 1, Member D1 (AKR1D1) (AA 1-326) protein (Strep Tag)Crystallography grade AKR1D1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Aldo-Keto Reductase Family 1, Member D1 (AKR1D1) (AA 1-325) protein (Strep Tag)Crystallography grade AKR1D1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Aldo-Keto Reductase Family 1, Member D1 (AKR1D1) protein (Myc-DYKDDDDK Tag)AKR1D1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.5) containing 1 mM DTT, 0.1M NaCl, 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- AKR1D1 (Aldo-Keto Reductase Family 1, Member D1 (AKR1D1))
- Alternative Name
- AKR1D1 (AKR1D1 Products)
- Synonyms
- 3o5bred Protein, CBAS2 Protein, SRD5B1 Protein, 3-oxo-5-beta-steroid 4-dehydrogenase-like Protein, aldo-keto reductase family 1 member D1 Protein, aldo-keto reductase family 1, member D1 Protein, aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase) Protein, LOC491379 Protein, AKR1D1 Protein, Akr1d1 Protein
- Background
- AKR1D1 also known as 3-oxo-5beta-steroid 4-deghydrogenase isoform 1, is a member of the AKR superfamily. The AKR family of proteins are soluble NADPH oxidoreductases. They play important roles in the metabolism of drugs, carcinogens and reactive aldehydes. AKR1D1 is responsible for the catalysis of the 5-betareduction of bile acid intermediates and steroid hormones which carry a delta (4)-3-one structure. AKR1D1 is highly expressed in liver, colon and testis. Deficiency of this enzyme may contribute to hepatic dysfunction. Recombinant AKR1D1 protein was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 37.3 kDa (326aa)
- NCBI Accession
- NP_005980
- UniProt
- P51857
- Pathways
- Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Monocarboxylic Acid Catabolic Process
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