P4HB Protein (AA 20-509) (His tag)

Catalog No. ABIN5854508

Product Details

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 20-509

Origin: Mouse

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This P4HB protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: DALEEEDNVL VLKKSNFEEA LAAHKYLLVE FYAPWCGHCK ALAPEYAKAA AKLKAEGSEI RLAKVDATEE SDLAQQYGVR GYPTIKFFKN GDTASPKEYT AGREADDIVN WLKKRTGPAA TTLSDTAAAE SLVDSSEVTV IGFFKDVESD SAKQFLLAAE AIDDIPFGIT SNSGVFSKYQ LDKDGVVLFK KFDEGRNNFE GEITKEKLLD FIKHNQLPLV IEFTEQTAPK IFGGEIKTHI LLFLPKSVSD YDGKLSSFKR AAEGFKGKIL FIFIDSDHTD NQRILEFFGL KKEECPAVRL ITLEEEMTKY KPESDELTAE KITEFCHRFL EGKIKPHLMS QEVPEDWDKQ PVKVLVGANF EEVAFDEKKN VFVEFYAPWC GHCKQLAPIW DKLGETYKDH ENIIIAKMDS TANEVEAVKV HSFPTLKFFP ASADRTVIDY NGERTLDGFK KFLESGGQDG AGDDEDLDLE EALEPDMEED DDQKAVKDEL LEHHHHHH

Purity: > 95 % by SDS - PAGE

Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Biological Activity Comment: Specific activity is >120 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650nm resulting from the reduction of insulin.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Bioactivity Validated

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Alternative Name: P4HB (P4HB Products)

Synonyms: DSI Protein, ERBA2L Protein, GIT Protein, P4Hbeta Protein, PDI Protein, PDIA1 Protein, PHDB Protein, PO4DB Protein, PO4HB Protein, PROHB Protein, PDA2 Protein, PDIP Protein, PDIR Protein, ERp59 Protein, Pdia1 Protein, Thbp Protein, p55 Protein, XPDIp Protein, pdi Protein, 1810041F13Rik Protein, AI661267 Protein, Pdip Protein, Pdipl Protein, p58 Protein, prolyl 4-hydroxylase subunit beta Protein, protein disulfide isomerase family A member 2 Protein, prolyl 4-hydroxylase, beta polypeptide Protein, protein disulfide isomerase family A member 2 L homeolog Protein, protein disulfide isomerase associated 2 Protein, prolyl 4-hydroxylase subunit beta L homeolog Protein, protein disulfide-isomerase Protein, P4HB Protein, PDIA2 Protein, P4hb Protein, pdia2.L Protein, Pdia2 Protein, p4hb.L Protein, LOC8281065 Protein

Background: P4HB, also known as protein disulfide-isomerase, prolyl 4-hydroxylase subunit beta, protocollagen hydroxylase, cellular thyroid hormone binding protein p55 and glutathione-insulin transhydrogenase, is an abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. At the cell surface, it seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. Recombinant mouse P4HB, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molecular Weight: 56.1kDa (498aa) 50-70KDa (SDS-PAGE under reducing conditions)

NCBI Accession: NP_035162

UniProt: P09103

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism