CPA4 Protein (AA 17-421) (His tag)
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- Target See all CPA4 Proteins
- CPA4 (Carboxypeptidase A4 (CPA4))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 17-421
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This CPA4 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- GQEKFFGDQV LRINVRNGDE ISKLSQLVNS NNLKLNFWKS PSSFNRPVDV LVPSVSLQAF KSFLRSQGLE YAVTIEDLQA LLDNEDDEMQ HNEGQERSSN NFNYGAYHSL EAIYHEMDNI AADFPDLARR VKIGHSFENR PMYVLKFSTG KGVRRPAVWL NAGIHSREWI SQATAIWTAR KIVSDYQRDP AITSILEKMD IFLLPVANPD GYVYTQTQNR LWRKTRSRNP GSSCIGADPN RNWNASFAGK GASDNPCSEV YHGPHANSEV EVKSVVDFIQ KHGNFKGFID LHSYSQLLMY PYGYSVKKAP DAEELDKVAR LAAKALASVS GTEYQVGPTC TTVYPASGSS IDWAYDNGIK FAFTFELRDT GTYGFLLPAN QIIPTAEETW LGLKTIMEHV RDNLYLEHHH HHH
- Purity
- > 95 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Top Product
- Discover our top product CPA4 Protein
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Carboxypeptidase A4 (CPA4) (AA 260-361) protein (GST tag)
CPA4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Carboxypeptidase A4 (CPA4) (AA 114-421) protein (His tag)Crystallography grade CPA4 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Carboxypeptidase A4 (CPA4) (AA 114-420) protein (His tag)Crystallography grade CPA4 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Carboxypeptidase A4 (CPA4) protein (Myc-DYKDDDDK Tag)CPA4 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- CPA4 (Carboxypeptidase A4 (CPA4))
- Alternative Name
- CPA4 (CPA4 Products)
- Synonyms
- zgc:91794 Protein, wu:fb66g10 Protein, CPA4 Protein, 1110019K20Rik Protein, AV009555 Protein, RGD1563619 Protein, CPA3 Protein, carboxypeptidase A4 Protein, cpa4 Protein, CPA4 Protein, MCYG_01475 Protein, VDBG_09090 Protein, MGYG_04594 Protein, Tsp_01317 Protein, Cpa4 Protein
- Background
- CPA4, also known as carboxypeptidase A4, is a secreted, zinc-dependent metallocarboxypeptidase that removes the C-terminal amino acid from peptides having a free C-terminal carboxyl group. CPA4 is synthesized as zymogens that are activated by proteolytic cleavage. Recombinant human CPA4, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 46.6kDa (413aa) 40-57KDa (SDS-PAGE under reducing conditions.)
- NCBI Accession
- NP_057436
- UniProt
- Q9UI42
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