AER61 Protein (AA 20-527) (His tag)
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- Target See all AER61 (C3orf64) Proteins
- AER61 (C3orf64) (Chromosome 3 Open Reading Frame 64 (C3orf64))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 20-527
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Origin
- Mouse
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This AER61 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- DKAHSEADDA PGKALYDYSS LRLPAEHIPF FLHNNRHVAS VCREDSHCPY KKHLENLNYC WGYEKSCAPE FRFGSPVCSY VDLGWTDTLE SAQDMFWRQA DFGYARERLG EIRTICQPER ASDSSLVCSR YLQYCRATGL YLDLRNIKRN HDRFKEDFLQ GGEIGGYCKL DSHALVSEGQ RKSPLQSWFA ELQGYTQLNF RPIEDAKCDI VVEKPTYFMK LDAGINMYHH FCDFLNLYLT QHVNNSFSTD VYIVMWDTST YGYGDLFSDT WKAFTDYDVI HLKTYDSKKV CFKEAVFSLL PRMRYGLFYN TPLISGCQNT GLFRAFSQHV LHRLNITQEG PKDGKVRVTI LARSTEYRKI LNQDELVNAL KTVSTFEVRV VDYKYRELGF LDQLRITHNT DIFIGMHGAG LTHLLFLPDW AAVFELYNCE DERCYLDLAR LRGIHYITWR KPSKVFPQDK GHHPTLGEHP KFTNYSFDVE EFMYLVLQAA EHVLQHPQWP FKKKHDELLE HHHHHH
- Purity
- > 85 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Top Product
- Discover our top product C3orf64 Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- AER61 (C3orf64) (Chromosome 3 Open Reading Frame 64 (C3orf64))
- Alternative Name
- Eogt (C3orf64 Products)
- Synonyms
- AER61 Protein, aer61 Protein, c3orf64 Protein, C12H3orf64 Protein, EOGT Protein, C22H3orf64 Protein, AOS4 Protein, C3orf64 Protein, EOGT1 Protein, A130022J15Rik Protein, AI447490 Protein, AW214473 Protein, AW259391 Protein, Aer61 Protein, EGF domain specific O-linked N-acetylglucosamine transferase Protein, glycosyltransferase aer61 Protein, EGF domain-specific O-linked N-acetylglucosamine (GlcNAc) transferase Protein, EGF domain-specific O-linked N-acetylglucosamine (GlcNAc) transferase L homeolog Protein, EOGT Protein, aer61 Protein, eogt Protein, eogt.L Protein, Eogt Protein
- Background
- EOGT, also known as EGF domain-specific O-linked N-acetylglucosamine transferase, is involved in the regulation of Notch receptor. O-GlcNAc (O-linked b-N-acetylglucosamine) is introduced by a single intracellular O-GlcNAc transferase (OGT) and a single extracellular O-GlcNAc transferase (EOGT). O-GlcNAc results from the addition of a single N-acetylglucosamine residue to serine/threonine residues. Recombinant mouse EOGT, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 60.4kDa (516aa) 50-70KDa (SDS-PAGE under reducing conditions.)
- NCBI Accession
- NP_780522
- UniProt
- Q8BYW9
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