IL-3 Protein (AA 20-152) (His tag)

Catalog No. ABIN7529333

Product Details

Target: IL-3 (Interleukin 3 (IL-3))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 20-152

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This IL-3 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: APMTQTTSLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIFLEHHHHH H

Purity: > 90 % by SDS - PAGE

Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Biological Activity Comment: Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 for this effects less or equal to 1.5 ng/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Bioactivity Validated

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: IL-3 (Interleukin 3 (IL-3))

Alternative Name: IL3 (IL-3 Products)

Synonyms: BPA Protein, Csfmu Protein, HCGF Protein, Il-3 Protein, MCGF Protein, PSF Protein, IL-3 Protein, MULTI-CSF Protein, interleukin 3 Protein, interleukin 3 (colony-stimulating factor, multiple) Protein, Il3 Protein, IL3 Protein

Background: IL3, as known as interleukin 3, is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. Also, this protein affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor, P-cell stimulation factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factors and WEHI-3 growth factor. Recombinant human IL3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molecular Weight: 16.1kDa (141aa) 13.5-18kDa (SDS-PAGE under reducing conditions)

NCBI Accession: NP_000579

UniProt: P08700

Pathways: JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy