GZMK Protein (AA 27-264) (His tag)
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- Target See all GZMK Proteins
- GZMK (Granzyme K (Granzyme 3, Tryptase II) (GZMK))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 27-264
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This GZMK protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- ADPIIGGKEV SPHSRPFMAS IQYGGHHVCG GVLIDPQWVL TAAHCQYRFT KGQSPTVVLG AHSLSKNEAS KQTLEIKKFI PFSRVTSDPQ SNDIMLVKLQ TAAKLNKHVK MLHIRSKTSL RSGTKCKVTG WGATDPDSLR PSDTLREVTV TVLSRKLCNS QSYYNGDPFI TKDMVCAGDA KGQKDSCKGD SGGPLICKGV FHAIVSGGHE CGVATKPGIY TLLTKKYQTW IKSNLVPPHT NHHHHHH
- Purity
- > 95 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Top Product
- Discover our top product GZMK Protein
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Granzyme K (Granzyme 3, Tryptase II) (GZMK) (AA 1-264) protein (GST tag)
GZMK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Granzyme K (Granzyme 3, Tryptase II) (GZMK) (AA 27-264) protein (His tag)Crystallography grade GZMK Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Granzyme K (Granzyme 3, Tryptase II) (GZMK) (AA 26-263) protein (His tag)Crystallography grade GZMK Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 20 % glycerol, 1 mM DTT.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- GZMK (Granzyme K (Granzyme 3, Tryptase II) (GZMK))
- Alternative Name
- GZMK (GZMK Products)
- Synonyms
- TRYP2 Protein, granzyme K Protein, GZMK Protein, Gzmk Protein
- Background
- GZMK, as known as Granzyme K, is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes. Recombinant human GZMK, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 26.9kDa (247aa) 28-40kDa (SDS-PAGE under reducing conditions.)
- NCBI Accession
- NP_002095
- UniProt
- P49863
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