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SRSF1 Protein (AA 1-248) (His tag)

SRSF1 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 95 % by SDS - PAGE SDS
Catalog No. ABIN5854856
  • Target See all SRSF1 Proteins
    SRSF1 (serine/arginine-Rich Splicing Factor 1 (SRSF1))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-248
    Origin
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 4
    • 4
    • 1
    • 1
    Baculovirus infected Insect Cells
    Purification tag / Conjugate
    This SRSF1 protein is labelled with His tag.
    Application
    SDS-PAGE (SDS)
    Sequence
    MSGGGVIRGP AGNNDCRIYV GNLPPDIRTK DIEDVFYKYG AIRDIDLKNR RGGPPFAFVE FEDPRDAEDA VYGRDGYDYD GYRLRVEFPR SGRGTGRGGG GGGGGGAPRG RYGPPSRRSE NRVVVSGLPP SGSWQDLKDH MREAGDVCYA DVYRDGTGVV EFVRKEDMTY AVRKLDNTKF RSHEGETAYI RVKVDGPRSP SYGRSRSRSR SRSRSRSRSN SRSRSYSPRR SRGSPRYSPR HSRSRSRTHH HHHH
    Purity
    > 95 % by SDS - PAGE
    Endotoxin Level
    < 1.0 EU per 1 microgram of protein (determined by LAL method)
    Top Product
    Discover our top product SRSF1 Protein
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.25 mg/mL
    Buffer
    Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 100  mM KCl, 1  mM DTT, 0.2  mM EDTA, 40 % Glycerol
    Storage
    4 °C,-20 °C,-80 °C
    Storage Comment
    Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
  • Target
    SRSF1 (serine/arginine-Rich Splicing Factor 1 (SRSF1))
    Alternative Name
    SRSF1 (SRSF1 Products)
    Synonyms
    ASF Protein, SF2 Protein, SF2p33 Protein, SFRS1 Protein, SRp30a Protein, 1110054N12Rik Protein, 5730507C05Rik Protein, 6330415C05Rik Protein, AI482334 Protein, AW491331 Protein, Asf Protein, Sf2 Protein, Sfrs1 Protein, DKFZp469K2419 Protein, sfrs1 Protein, sfrs1b Protein, wu:fb80g05 Protein, zgc:111894 Protein, zgc:65898 Protein, zgc:76897 Protein, asf Protein, sf2 Protein, sf2p33 Protein, srp30a Protein, sfrs1a Protein, sfrs1l Protein, wu:fb52g10 Protein, wu:fb97g12 Protein, zgc:66146 Protein, serine and arginine rich splicing factor 1 Protein, serine/arginine-rich splicing factor 1 Protein, serine/arginine-rich splicing factor 1b Protein, serine/arginine-rich splicing factor 1 L homeolog Protein, serine/arginine-rich splicing factor 1a Protein, SRSF1 Protein, Srsf1 Protein, srsf1b Protein, srsf1.L Protein, srsf1a Protein
    Background
    SRSF1, also known as Serine/arginine-rich splicing factor 1, is a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. This protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. In addition to being involved in the splicing process, it also mediates post-splicing activities, such as mRNA nuclear export and translation. Recombinant human SRSF1 protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
    Molecular Weight
    28.5kDa (254aa) 28-40KDa (SDS-PAGE under reducing conditions.)
    NCBI Accession
    NP_008855
    UniProt
    Q07955
    Pathways
    Ribonucleoprotein Complex Subunit Organization
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