MCAM Protein (AA 24-559) (His tag)
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- Target See all MCAM Proteins
- MCAM (Melanoma Cell Adhesion Molecule (MCAM))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 24-559
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This MCAM protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- VPGEAEQPAP ELVEVEVGST ALLKCGLSQS QGNLSHVDWF SVHKEKRTLI FRVRQGQGQS EPGEYEQRLS LQDRGATLAL TQVTPQDERI FLCQGKRPRS QEYRIQLRVY KAPEEPNIQV NPLGIPVNSK EPEEVATCVG RNGYPIPQVI WYKNGRPLKE EKNRVHIQSS QTVESSGLYT LQSILKAQLV KEDKDAQFYC ELNYRLPSGN HMKESREVTV PVFYPTEKVW LEVEPVGMLK EGDRVEIRCL ADGNPPPHFS ISKQNPSTRE AEEETTNDNG VLVLEPARKE HSGRYECQGL DLDTMISLLS EPQELLVNYV SDVRVSPAAP ERQEGSSLTL TCEAESSQDL EFQWLREETG QVLERGPVLQ LHDLKREAGG GYRCVASVPS IPGLNRTQLV NVAIFGPPWM AFKERKVWVK ENMVLNLSCE ASGHPRPTIS WNVNGTASEQ DQDPQRVLST LNVLVTPELL ETGVECTASN DLGKNTSILF LELVNLTTLT PDSNTTTGLS TSTASPHTRA NSTSTERKLP EPESRGAAAL EHHHHHH
- Purity
- > 95 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1ug of protein (determined by LAL method)
- Top Product
- Discover our top product MCAM Protein
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Melanoma Cell Adhesion Molecule (MCAM) (AA 24-559) protein (His tag)
MCAM Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Melanoma Cell Adhesion Molecule (MCAM) (AA 24-563) protein (His tag)MCAM Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Melanoma Cell Adhesion Molecule (MCAM) (AA 1-646) protein (GST tag)MCAM Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Melanoma Cell Adhesion Molecule (MCAM) (AA 24-646) protein (His-DYKDDDDK-Strep II Tag)MCAM Origin: Human Host: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
Melanoma Cell Adhesion Molecule (MCAM) (AA 24-559) protein (His tag)Crystallography grade MCAM Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Melanoma Cell Adhesion Molecule (MCAM) (AA 24-563) protein (His tag)Crystallography grade MCAM Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Melanoma Cell Adhesion Molecule (MCAM) (AA 24-646) protein (rho-1D4 tag)Crystallography grade MCAM Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Melanoma Cell Adhesion Molecule (MCAM) (AA 24-648) protein (rho-1D4 tag)Crystallography grade MCAM Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- MCAM (Melanoma Cell Adhesion Molecule (MCAM))
- Alternative Name
- MCAM (MCAM Products)
- Synonyms
- CD146 Protein, MUC18 Protein, Muc18 Protein, 1-gicerin Protein, AV025631 Protein, CD149 Protein, s-endo Protein, s-gicerin Protein, cd146 Protein, gicerin Protein, id:ibd3512 Protein, mcam Protein, MCAM Protein, melanoma cell adhesion molecule Protein, melanoma cell adhesion molecule b Protein, MCAM Protein, Mcam Protein, mcamb Protein
- Background
- MCAM, also known as cell surface glycoprotein MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. It is associated with various carcinomas such as tumor progression, metastasis and may be involved in embryonic neural development. This protein also plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Recombinant human MCAM protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 61.0kDa (547aa) 70-100KDa (SDS-PAGE under reducing conditions.)
- NCBI Accession
- NP_006491
- UniProt
- P43121
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