PPID Protein (AA 1-370) (His tag)

Catalog No. ABIN7281328

This Recombinant PPID protein is expressed in Escherichia coli (E. coli).

Quick Overview for PPID Protein (AA 1-370) (His tag) (ABIN7281328)

Target: PPID (Peptidylprolyl Isomerase D (PPID))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 90 % by SDS - PAGE

Product Details

Protein Characteristics: AA 1-370

Purification tag / Conjugate: This PPID protein is labelled with His tag.

Sequence: MGSSHHHHHH SSGLVPRGSH MGSEFMSHAS PAAKPSNSKN PRVFFDVDIG GERVGRIVLE LFADIVPKTA ENFRALCTGE KGTGSTTGKP LHFKGCPFHR IIKKFMIQGG DFSNQNGTGG ESIYGEKFED ENFHYKHDRE GLLSMANAGP NTNGSQFFIT TVPTPHLDGK HVVFGQVIKG LGVARTLENV EVNGEKPAKL CVIAECGELK EGDDWGIFPK DGSGDSHPDF PEDADIDLKD VDKILLISED LKNIGNTFFK SQNWEMAIKK YAKVLRYVDS SKAVIEKADR SRLQPIALSC VLNIGACKLK MSNWQGAIDS CLEALEMDPS NTKALYRKAQ GWQGLKEYDQ ALADLKKAQE IAPGDKAIQA ELLKVKQMIK AQKDKEKAVY AKMFA

Biological Activity Comment: Specific activity is > 700nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAFP-PNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Bioactivity Validated

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In 20  mM Tris-Hcl buffer (  pH 8.0) containing 10 % glycerol, 1  mM DTT

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: PPID (Peptidylprolyl Isomerase D (PPID))

Alternative Name: Ppid

Background: Ppid, also known as Peptidyl-prolyl cis-trans isomerase D, is a member of peptidyl-propyl cis-trans isomerase (Pplase) family, which catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. This protein can bind to the immunosuppressant cyclosporine A and was known that its overexpression the apoptosis in caner cells. Recombinant mouse Ppid protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 43.4 kDa (395aa) confirmed by MALDI-TOF

NCBI Accession: NP_080628

UniProt: Q9CR16

Pathways: Nuclear Hormone Receptor Binding