PPID Protein (AA 1-370) (His tag)
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- Target See all PPID Proteins
- PPID (Peptidylprolyl Isomerase D (PPID))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-370
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PPID protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSEFMSHAS PAAKPSNSKN PRVFFDVDIG GERVGRIVLE LFADIVPKTA ENFRALCTGE KGTGSTTGKP LHFKGCPFHR IIKKFMIQGG DFSNQNGTGG ESIYGEKFED ENFHYKHDRE GLLSMANAGP NTNGSQFFIT TVPTPHLDGK HVVFGQVIKG LGVARTLENV EVNGEKPAKL CVIAECGELK EGDDWGIFPK DGSGDSHPDF PEDADIDLKD VDKILLISED LKNIGNTFFK SQNWEMAIKK YAKVLRYVDS SKAVIEKADR SRLQPIALSC VLNIGACKLK MSNWQGAIDS CLEALEMDPS NTKALYRKAQ GWQGLKEYDQ ALADLKKAQE IAPGDKAIQA ELLKVKQMIK AQKDKEKAVY AKMFA
- Purity
- > 90 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 700nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAFP-PNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin
- Top Product
- Discover our top product PPID Protein
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Peptidylprolyl Isomerase D (PPID) (AA 1-370) protein (GST tag)
PPID Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Peptidylprolyl Isomerase D (PPID) (AA 2-370) protein (His tag)PPID Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Peptidylprolyl Isomerase D (PPID) (AA 1-370) protein (His tag)PPID Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Peptidylprolyl Isomerase D (PPID) (AA 1-370) (Active) protein (His tag)PPID Origin: Human Host: Escherichia coli (E. coli) Recombinant SDS, Func Active
Peptidylprolyl Isomerase D (PPID) (AA 2-370) protein (His tag)Crystallography grade PPID Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Peptidylprolyl Isomerase D (PPID) (AA 2-370) protein (His tag)Crystallography grade PPID Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-Hcl buffer ( pH 8.0) containing 10 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- PPID (Peptidylprolyl Isomerase D (PPID))
- Alternative Name
- Ppid (PPID Products)
- Synonyms
- PPID Protein, CYP-40 Protein, CYPD Protein, Cyp-40 Protein, CypD Protein, wu:fb18b07 Protein, zgc:86711 Protein, 4930564J03Rik Protein, Ppidl Protein, Ppif Protein, cyp-40 Protein, cypd Protein, peptidylprolyl isomerase D Protein, peptidylprolyl isomerase D (cyclophilin D) Protein, peptidylprolyl isomerase D (cyclophilin D) S homeolog Protein, PPID Protein, Ppid Protein, ppid Protein, ppid.S Protein
- Background
- Ppid, also known as Peptidyl-prolyl cis-trans isomerase D, is a member of peptidyl-propyl cis-trans isomerase (Pplase) family, which catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. This protein can bind to the immunosuppressant cyclosporine A and was known that its overexpression the apoptosis in caner cells. Recombinant mouse Ppid protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 43.4 kDa (395aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_080628
- UniProt
- Q9CR16
- Pathways
- Nuclear Hormone Receptor Binding
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