HCST Protein (AA 20-48) (hIgG-His-tag)
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- Target See all HCST Proteins
- HCST (Hematopoietic Cell Signal Transducer (HCST))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 20-48
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This HCST protein is labelled with hIgG-His-tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MHRHHPREMK KRVEDLHVGA TVAPSSRRDF TFDLYRALAS AAPSQNIFFS PVSISMSLAM LSLGAGSSTK MQILEGLGLN LQKSSEKELH RGFQQLLQEL NQPRDGFQLS LGNALFTDLV VDLQDTFVSA MKTLYLADTF PTNFRDSAGA MKQINDYVAK QTKGKIVDLL KNLDSNAVVI MVNYIFFKAK WETSFNHKGT QEQDFYVTSE TVVRVPMMSR EDQYHYLLDR NLSCRVVGVP YQGNATALFI LPSEGKMQQV ENGLSEKTLR KWLKMFKKRQ LELYLPKFSI EGSYQLEKVL PSLGISNVFT SHADLSGISN HSNIQVSEMV HKAVVEVDES GTRAAAATGT IFTFRSARLN SQRLVFNRPF LMFIVDNNIL FLGKVNRP
- Purity
- > 90 % by SDS - PAGE
- Biological Activity Comment
- Measured by its ability to inhibit Thrombin cleavage of substrate Boc-VPR-AMC. The IC50 for this effect is less or equal to 2 nM
- Top Product
- Discover our top product HCST Protein
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Hematopoietic Cell Signal Transducer (HCST) (AA 19-93) protein (rho-1D4 tag)
Crystallography grade HCST Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, Crys, ELISA, WB
Hematopoietic Cell Signal Transducer (HCST) (AA 18-79) protein (rho-1D4 tag)Crystallography grade HCST Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, Crys, ELISA, WB
Hematopoietic Cell Signal Transducer (HCST) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)HCST Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM MES buffer ( pH 6.0) containing 150 mM NaCl, 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- HCST (Hematopoietic Cell Signal Transducer (HCST))
- Alternative Name
- HCST (HCST Products)
- Synonyms
- DAP10 Protein, KAP10 Protein, PIK3AP Protein, Dap10 Protein, HCST Protein, dap10 Protein, dap10-A Protein, hcst Protein, hcsta Protein, kap10 Protein, pik3ap Protein, hematopoietic cell signal transducer Protein, hematopoietic cell signal transducer L homeolog Protein, HCST Protein, Hcst Protein, hcst Protein, hcst.L Protein
- Background
- Serpin A5, also known as PCI (Protein C Inhibitor) or PAI3 (plasminogen activator inhibitor 3), is a member of the serpin serine proteinase inhibitor family. This protein inhibits plasminogen activators as well as activated protein C. Serpin A5 is secreted in plasma, but is also expressed in liver. Serpin A5 is involved in cell inflammation, proliferation, apoptosis, tumor cell migration, invasion, and metastasis. Recombinant Serpin A5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 45.9 kDa (408aa), confirmed by MALDI-TOF
- UniProt
- P05154
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