SETD8 Protein (AA 195-352) (His tag)
-
- Target See all SETD8 Proteins
- SETD8 (SET Domain Containing (Lysine Methyltransferase) 8 (SETD8))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 195-352
-
Origin
- Human
-
Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This SETD8 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MKAELQSEER KRIDELIESG KEEGMKIDLI DGKGRGVIAT KQFSRGDFVV EYHGDLIEIT DAKKREALYA QDPSTGCYMY YFQYLSKTYC VDATRETNRL GRLINHSKCG NCQTKLHDID GVPHLILIAS RDIAAGEELL YDYGDRSKAS IEAHPWLKHH HHHHH
- Purity
- > 85 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1ug of protein (determined by LAL method)
- Top Product
- Discover our top product SETD8 Protein
-
SET Domain Containing (Lysine Methyltransferase) 8 (SETD8) (AA 195-352) protein (His tag)
SETD8 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
SET Domain Containing (Lysine Methyltransferase) 8 (SETD8) (AA 1-393) protein (Strep Tag)Crystallography grade SETD8 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
SET Domain Containing (Lysine Methyltransferase) 8 (SETD8) (AA 1-349) protein (Strep Tag)Crystallography grade SETD8 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 5 mM DTT, 0.2M NaCl, 1 mM EDTA, 50 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
-
- Target
- SETD8 (SET Domain Containing (Lysine Methyltransferase) 8 (SETD8))
- Alternative Name
- KMT5A (SETD8 Products)
- Synonyms
- KMT5A Protein, PR-Set7 Protein, SET07 Protein, SET8 Protein, 2410195B05Rik Protein, AA617402 Protein, AW536475 Protein, PR-SET7 Protein, setd8b Protein, wu:fc23f12 Protein, zgc:171649 Protein, Pr-set7 Protein, RGD1305893 Protein, Setd8 Protein, kmt5a Protein, kmt5a-a Protein, setd8 Protein, setd8-a Protein, set8a Protein, setd8a Protein, wu:fj33f02 Protein, zgc:153719 Protein, lysine methyltransferase 5A Protein, lysine methyltransferase 5Ab Protein, lysine methyltransferase 5A S homeolog Protein, lysine methyltransferase 5Aa Protein, KMT5A Protein, Kmt5a Protein, kmt5ab Protein, kmt5a.S Protein, kmt5aa Protein
- Background
- KMT5A, also known as N-lysine methyltransferase KMT5A isoform 1, is an enzyme which catalyzes mono-methylation of histone H4 at lysine 20 (H4K20me1). It is implicated in cell-cycle-dependent transcriptional silencing and mitotic regulation in metazoans. It acts as a barrier to prevent cellular senescence through chromatin-mediated regulation of senescence-associated metabolic remodeling. It mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. The loss of KMT5A concurrently stimulate nucleolar function and retinoblastoma protein-mediated mitochondrial metabolism. Recombinant human KMT5A, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 18.9kDa (165aa) 18-28kDa (SDS-PAGE under reducing conditions)
- NCBI Accession
- NP_065115
- UniProt
- Q9NQR1
-
