SMAD3 Protein (AA 2-425) (DYKDDDDK Tag,His tag)

Catalog No. ABIN7314542

Product Details

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Protein Type: Recombinant

Protein Characteristics: AA 2-425

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This SMAD3 protein is labelled with DYKDDDDK Tag,His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Mass Spectrometry (MS)

Purpose: Recombinant Human SMAD3 Protein is produced by E.coli expression system and the target gene encoding Ser2-Ser425 is expressed with a 6His, Flag tag at the N-terminus.

Sequence: MRGSHHHHHH GSDYKDDDDK SSILPFTPPI VKRLLGWKKG EQNGQEEKWC EKAVKSLVKK LKKTGQLDEL EKAITTQNVN TKCITIPRSL DGRLQVSHRK GLPHVIYCRL WRWPDLHSHH ELRAMELCEF AFNMKKDEVC VNPYHYQRVE TPVLPPVLVP RHTEIPAEFP PLDDYSHSIP ENTNFPAGIE PQSNIPETPP PGYLSEDGET SDHQMNHSMD AGSPNLSPNP MSPAHNNLDL QPVTYCEPAF WCSISYYELN QRVGETFHAS QPSMTVDGFT DPSNSERFCL GLLSNVNRNA AVELTRRHIG RGVRLYYIGG EVFAECLSDS AIFVQSPNCN QRYGWHPATV CKIPPGCNLK IFNNQEFAAL LAQSVNQGFE AVYQLTRMCT IRMSFVKGWG AEYRRQTVTS TPCWIELHLN GPLQWLDKVL TQMGSPSIRC SSVS

Purity: Greater than 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20  mM PB, 500  mM NaCl,  pH 7.5.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Target Details

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Alternative Name: SMAD3 (SMAD3 Products)

Synonyms: HSPC193 Protein, HsT17436 Protein, JV15-2 Protein, LDS1C Protein, LDS3 Protein, MADH3 Protein, madh3 Protein, madh3a Protein, smad3 Protein, wu:fa99e03 Protein, XSmad3 Protein, XenMLP Protein, Xmad3 Protein, madh3-A Protein, Madh3 Protein, AU022421 Protein, Smad 3 Protein, mad3 Protein, madh3b Protein, zgc:92234 Protein, SMAD family member 3 Protein, SMAD family member 3a Protein, SMAD family member 3 L homeolog Protein, SMAD family member 3b Protein, SMAD3 Protein, smad3a Protein, smad3.L Protein, Smad3 Protein, smad3b Protein

Gene ID: 4088

UniProt: P84022

Pathways: Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy