Growth Hormone 1 Protein (GH1)

Catalog No. ABIN621622

Product Details

Target: Growth Hormone 1 (GH1)

Protein Type: Recombinant

Origin: Cow

Host: Please inquire

Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Phe-Pro-Ala.

Characteristics: Recombinant Bovine Growth Hormone

Purity: > 98.0 % as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.68 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program o

Buffer: The Bovine Growth Hormone protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO 3 adjusted to pH-8.It is recommended to reconstitute the lyophilized Bovine Growth Hormone in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Storage: -20 °C

References

Wang, Ji, Wang, Chen, Wang, Loor: "The preliminary study on the effects of growth hormone and insulin-like growth factor-I on κ-casein synthesis in bovine mammary epithelial cells in vitro." in: Journal of animal physiology and animal nutrition, Vol. 100, Issue 2, pp. 251-5, (2016) (PubMed).

Target Details

Target: Growth Hormone 1 (GH1)

Alternative Name: Growth Hormone (GH1 Products)

Background: Growth Hormone Bovine Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 191 amino acids and having a molecular mass of 21.8 kDa. The GH Bovine Recombinant is purified by proprietary chromatographic techniques. Introduction: GH is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation, an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature. Synonyms: BGH, BST, rBGH, rBST, Bovine Somatotropin, Bovine GH, Growth hormone, GH1.

Pathways: NF-kappaB Signaling, JAK-STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process