GM-CSF Protein (AA 18-144) (His tag)
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- Target See all GM-CSF (CSF2) Proteins
- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
- Biological Activity
- Active
- Protein Characteristics
- AA 18-144
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This GM-CSF protein is labelled with His tag.
- Application
- Cell Culture (CC), Activity Assay (AcA)
- Characteristics
- Tag location: N-terminal His Tag
- Purity
- > 95 %
- Biological Activity Comment
- TF-1, the human erythroleukemia cell line,provides a good system for detecting the activity of GM-CSF for it is a cell line of immature erythroid origin that completely depends on interleukin 3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF) for long term growth. As reported, GM-CSF was also able to induce differentiation of human monoblastic leukemia cell line U937. In house data was obtained by the following experiment: TF-1 cells and U937 cells were incubated in the presence of various concentrations of rhGM-CSF, then cells were observed by inverted microscope everyday . Cell proliferation of TF1 cells after incubation with GM-CSF (10ng/mL) for 3 days was shown in Figure 1.
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- Discover our top product CSF2 Protein
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Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) protein
CSF2 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-141) (Active) proteinCSF2 Origin: Mouse Host: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (Active) proteinCSF2 Origin: Rat Host: Escherichia coli (E. coli) Recombinant SDS, Func Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (Active) protein (His tag)CSF2 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (Active) proteinCSF2 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) protein (His tag)CSF2 Origin: Rat Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 1-144) protein (GST tag)CSF2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) protein (His tag)Crystallography grade CSF2 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Application Notes
- Isoelectric Point: 5.2
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
- Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Target
- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
- Alternative Name
- Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) (CSF2 Products)
- Synonyms
- GMCSF Protein, GM-CSF Protein, CSF2 Protein, Csfgm Protein, Gm-CSf Protein, MGI-IGM Protein, Gm-csf Protein, Gmcsf Protein, CSF Protein, colony stimulating factor 2 Protein, colony stimulating factor 2 (granulocyte-macrophage) Protein, CSF2 Protein, Csf2 Protein
- Background
- Alternative Names: CSF2, GM-CSF, Sargramostim, Molgramostin, Granulocyte-Macrophage Colony Stimulating Factor
- Molecular Weight
- 19-26kDa
- UniProt
- P04141
- Pathways
- JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin
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