CD137 Protein (AA 40-153) (His tag)
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- Target See all CD137 (TNFRSF9) Proteins
- CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))
- Biological Activity
- Active
- Protein Characteristics
- AA 40-153
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This CD137 protein is labelled with His tag.
- Application
- Activity Assay (AcA), Cell Culture (CC)
- Characteristics
- Tag location: N-terminal His Tag
- Purity
- > 97 %
- Biological Activity Comment
- TNFRSF9 (Tumor necrosis factor receptor superfamily member 9) is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. A binding ELISA assay was conducted to detect the interaction of TNFRSF9 and TNFa. Briefly, recombinant human TNFRSF9 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-TNFRSF9 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF9 and TNFa was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of TNFRSF9 with TNFa.
- Top Product
- Discover our top product TNFRSF9 Protein
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Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 24-186) protein (Fc-Avi Tag)
TNFRSF9 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 24-186) protein (His tag)TNFRSF9 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 24-186) protein (His-Avi Tag,Biotin)TNFRSF9 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 24-186) protein (His tag)TNFRSF9 Origin: Cynomolgus, Rhesus Monkey Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 24-186) protein (Fc-Avi Tag,Biotin)TNFRSF9 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 24-186) protein (mFc-His Tag)TNFRSF9 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA, FACS
Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 24-211) protein (His tag)TNFRSF9 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 46-128) protein (His-DYKDDDDK-Strep II Tag)TNFRSF9 Origin: Human Host: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 24-186) protein (His-DYKDDDDK-Strep II Tag)TNFRSF9 Origin: Human Host: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9) (AA 24-211) protein (Fc Tag)TNFRSF9 Origin: Mouse Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE.
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- Application Notes
- Isoelectric Point: 8.3
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
- Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Target
- CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))
- Abstract
- TNFRSF9 Products
- Background
- Alternative Names: CD137, CDw137, 4-1BB, ILA, Induced By Lymphocyte Activation, T-cell antigen 4-1BB homolog
- Molecular Weight
- 16kDa
- UniProt
- Q07011
- Pathways
- Cancer Immune Checkpoints
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