Galectin 9 Protein (AA 1-355) (His tag,GST tag)

Catalog No. ABIN6239815

Product Details

Target: Galectin 9 (LGALS9) (Lectin, Galactoside-Binding, Soluble, 9 (LGALS9))

Biological Activity: Active

Protein Characteristics: AA 1-355

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Galectin 9 protein is labelled with His tag,GST tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Characteristics: Tag location: N-terminal His and GST Tag

Purity: > 90 %

Biological Activity Comment: GAL9 (Galectin-9) belongs to the galectin family, which is defined by their binding specificity for β-galactoside sugars, such as N-acetyllactosamine (Galβ1-3GlcNAc or Galβ1-4GlcNAc). It is reported that GAL9 induces T-helper type 1 lymphocyte (Th1) death by binding to HAVCR2 (Hepatitis A virus cellular receptor 2); besides, the interaction between GAL9 and PDI (Protein disulfide-isomerase) leads to disulfide reductase activity increasing at the plasma membrane, therefore alters the plasma membrane redox state and enhances cell migration. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human GAL9 with recombinant human HAVCR2 and recombinant human PDI separately. Briefly, GAL9 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HAVCR2-coated and PDI-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-GAL9 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of GAL9 with HAVCR2 and PDI were shown in Figure 1 and Figure 2, and this effect was in a dose dependent manner The binding activity of GAL9 with HAVCR2. Figure 2. The binding activity of GAL9 with PDI.

Application Details

Application Notes: Isoelectric Point: 9.3

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Target Details

Target: Galectin 9 (LGALS9) (Lectin, Galactoside-Binding, Soluble, 9 (LGALS9))

Alternative Name: Galectin 9 (GAL9) (LGALS9 Products)

Synonyms: huat Protein, lgals9a Protein, ecalectin Protein, galectin-9 Protein, Lgals9 Protein, wu:fd20c09 Protein, zgc:111833 Protein, LGALS9 Protein, AA407335 Protein, AI194909 Protein, AI265545 Protein, LGALS35 Protein, Lgals5 Protein, gal-9 Protein, HUAT Protein, LGALS9A Protein, UAT Protein, UATP.I Protein, galectin 9 L homeolog Protein, galectin 9 Protein, lectin, galactoside-binding, soluble, 9 (galectin 9)-like 3 Protein, lectin, galactose binding, soluble 9 Protein, lgals9.L Protein, lgals9 Protein, lgals9l3 Protein, LGALS9 Protein, Lgals9 Protein

Background: Alternative Names: LGALS9, Ecalectin, Lectin,Galactoside-Binding Soluble 9, Tumor antigen HOM-HD-21

Molecular Weight: 69kDa

UniProt: O00182