NEU1 Protein (AA 47-415) (His tag)
-
- Target See all NEU1 Proteins
- NEU1 (Sialidase 1 (Lysosomal Sialidase) (NEU1))
- Biological Activity
- Active
- Protein Characteristics
- AA 47-415
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NEU1 protein is labelled with His tag.
- Application
- Activity Assay (AcA), Cell Culture (CC)
- Characteristics
- Tag location: N-terminal His Tag
- Purity
- > 97 %
- Biological Activity Comment
- NEU (Sialidase-1) is an enzyme that catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A (CTSA). Thus a binding ELISA assay was conducted to detect the interaction of recombinant human NEU and recombinant human CTSA. Briefly, NEU were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CTSA-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-NEU pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of NEU and CTSA was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of NEU with CTSA.
- Top Product
- Discover our top product NEU1 Protein
-
Sialidase 1 (Lysosomal Sialidase) (NEU1) (AA 48-415) protein (His tag)
NEU1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Sialidase 1 (Lysosomal Sialidase) (NEU1) (AA 334-415) protein (GST tag)NEU1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Sialidase 1 (Lysosomal Sialidase) (NEU1) (AA 48-415) protein (His tag)Crystallography grade NEU1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Sialidase 1 (Lysosomal Sialidase) (NEU1) (AA 42-409) protein (His tag)Crystallography grade NEU1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Sialidase 1 (Lysosomal Sialidase) (NEU1) (AA 141-286) (Active) protein (His tag)NEU1 Origin: Human Host: Escherichia coli (E. coli) > 90 % AcA, CC Active
-
- Application Notes
- Isoelectric Point: 5.3
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
- Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
-
- Target
- NEU1 (Sialidase 1 (Lysosomal Sialidase) (NEU1))
- Alternative Name
- Neuraminidase (NEU) (NEU1 Products)
- Target Type
- Influenza Protein
- Background
- Alternative Names: NEU1, SIAL1, Sialidase 1, Lysosomal Sialidase, Acetylneuraminyl hydrolase, G9 sialidase, N-acetyl-alpha-neuraminidase 1
- Molecular Weight
- 46kDa
- UniProt
- Q99519
- Pathways
- SARS-CoV-2 Protein Interactome
-
