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FGF13 Protein (AA 1-192) (His tag)

FGF13 Origin: Rat Host: Escherichia coli (E. coli) > 97 % AcA, CC Active
Catalog No. ABIN6239865
  • Target See all FGF13 Proteins
    FGF13 (Fibroblast Growth Factor 13 (FGF13))
    Biological Activity
    Active
    Protein Characteristics
    AA 1-192
    Origin
    • 8
    • 3
    • 3
    • 1
    Rat
    Source
    • 12
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This FGF13 protein is labelled with His tag.
    Application
    Activity Assay (AcA), Cell Culture (CC)
    Characteristics
    Tag location: N-terminal His Tag
    Purity
    > 97 %
    Biological Activity Comment
    FGF13 (Fibroblast growth factor 13) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. A proliferation assay was conducted to detect the bioactivity of recombinant rat FGF13 using 3T3 cells. Briefly, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of FGF13. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of 3T3 cells after incubation with FGF13 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant FGF13 for 48h. The result was shown in Figure 2. It was obvious that FGF13 significantly increased cell viability of 3T3 cells.
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    Discover our top product FGF13 Protein
  • Application Notes
    Isoelectric Point: 9.2
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
    Preservative
    Dithiothreitol (DTT), Other preservative, ProClin
    Precaution of Use
    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • Target
    FGF13 (Fibroblast Growth Factor 13 (FGF13))
    Abstract
    FGF13 Products
    Synonyms
    FGF13 Protein, fgf2 Protein, fhf2 Protein, fgf13 Protein, FGF-13 Protein, xFGF13 Protein, FGF2 Protein, FHF-2 Protein, FHF2 Protein, Fhf2 Protein, zgc:101784 Protein, fibroblast growth factor 13 Protein, fibroblast growth factor 13 L homeolog Protein, fibroblast growth factor 13a Protein, FGF13 Protein, fgf13 Protein, fgf13.L Protein, Fgf13 Protein, fgf13a Protein
    Background
    Alternative Names: FHF2, Fibroblast Growth Factor Homologous Factor 2
    Molecular Weight
    24kDa
    UniProt
    Q9ERW3
    Pathways
    Regulation of Cell Size
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