CAPNL1 Protein (AA 75-356) (His tag)

Catalog No. ABIN6239882

Product Details

Target: CAPNL1 (Calpain 1, Large Subunit (CAPNL1))

Biological Activity: Active

Protein Characteristics: AA 75-356

Origin: Rat

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This CAPNL1 protein is labelled with His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Characteristics: Tag location: N-terminal His Tag

Purity: > 95 %

Biological Activity Comment: Calpain 1, Large Subunit (CAPN1) is an intracellular protease that requires calcium for its catalytic activity. Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. It has broad endopeptidase specificity. Besides, Signal Transducer And Activator Of Transcription 3 (STAT3) has been identified as an interactor of CAPN1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CAPN1 and recombinant rat STAT3. Briefly, CAPN1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to STAT3-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CAPN1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of CAPN1 and STAT3 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of CAPN1 with STAT3.

Application Details

Application Notes: Isoelectric Point: 5.3

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Target Details

Target: CAPNL1 (Calpain 1, Large Subunit (CAPNL1))

Alternative Name: Calpain 1, Large Subunit (CAPN1) (CAPNL1 Products)

Synonyms: CANP Protein, CANP1 Protein, CANPL1 Protein, muCANP Protein, muCL Protein, Capa-1 Protein, Capa1 Protein, mu-calpin Protein, si:ch211-203k12.4 Protein, si:dkeyp-101g1.3 Protein, calpain Protein, calpain 1 Protein, calpain 1, (mu/I) large subunit Protein, calpain 1, (mu/I) large subunit b Protein, calpain 11 Protein, CAPN1 Protein, Capn1 Protein, capn1 Protein, capn1b Protein, CAPN11 Protein

Background: Alternative Names: PIG30, CANPL1, MuCANP, MuCL, Calpain-1 Catalytic Subunit, Calcium-Dependent Protease 1, Calcium-activated neutral proteinase 1, Calpain mu-type, Micromolar-calpain

Molecular Weight: 33kDa

UniProt: P97571

Pathways: Apoptosis