PON3 Protein (AA 2-354) (His tag)
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- Target See all PON3 Proteins
- PON3 (Paraoxonase 3 (PON3))
- Biological Activity
- Active
- Protein Characteristics
- AA 2-354
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PON3 protein is labelled with His tag.
- Application
- Activity Assay (AcA), Cell Culture (CC)
- Characteristics
- Tag location: N-terminal His Tag
- Purity
- > 97 %
- Biological Activity Comment
- Paraoxonase 3 (PON3) is secreted into the bloodstream and associates with high-density lipoprotein (HDL). PON3 also rapidly hydrolyzes lactones and can inhibit the oxidation of low-density lipoprotein, a function that is believed to slow the initiation and progression of atherosclerosis. Besides, Paraoxonase 1 (PON1) has been identified as an interactor of PON3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PON3 and recombinant human PON1. Briefly, PON3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to PON1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-PON3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of PON3 and PON1 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of PON3 with PON1.
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- Discover our top product PON3 Protein
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Paraoxonase 3 (PON3) (AA 1-354) protein (GST tag)
PON3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Paraoxonase 3 (PON3) (AA 2-354) protein (His tag)Crystallography grade PON3 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Application Notes
- Isoelectric Point: 5.2
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
- Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Target
- PON3 (Paraoxonase 3 (PON3))
- Abstract
- PON3 Products
- Synonyms
- 2810004E20 Protein, AI786302 Protein, paraoxonase 3 Protein, PON3 Protein, Pon3 Protein
- Background
- Alternative Names: Serum paraoxonase/lactonase 3
- Molecular Weight
- 43kDa
- UniProt
- Q15166
- Pathways
- Monocarboxylic Acid Catabolic Process
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