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Arylsulfatase B Protein (ARSB) (AA 81-533) (His tag)

ARSB Origin: Human Host: Escherichia coli (E. coli) > 80 % CC, AcA Active
Catalog No. ABIN6239887
  • Target See all Arylsulfatase B (ARSB) Proteins
    Arylsulfatase B (ARSB)
    Biological Activity
    Active
    Protein Characteristics
    AA 81-533
    Origin
    • 7
    • 1
    • 1
    • 1
    Human
    Source
    • 3
    • 2
    • 2
    • 2
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This Arylsulfatase B protein is labelled with His tag.
    Application
    Cell Culture (CC), Activity Assay (AcA)
    Characteristics
    Tag location: N-terminal His Tag
    Purity
    > 80 %
    Biological Activity Comment
    Arylsulfatase B (ARSB) is a lysosomal enzyme of the sulfatase family. ARSB hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targetted to the lysozyme. Besides, Plasminogen Activator, Urokinase Receptor (uPAR) has been identified as an interactor of ARSB, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ARSB and recombinant human uPAR. Briefly, ARSB were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to uPAR-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-ARSB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of ARSB and uPAR was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of ARSB with uPAR.
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  • Application Notes
    Isoelectric Point: 7.7
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
    Preservative
    Dithiothreitol (DTT), Other preservative, ProClin
    Precaution of Use
    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • Target
    Arylsulfatase B (ARSB)
    Abstract
    ARSB Products
    Synonyms
    ASB Protein, G4S Protein, MPS6 Protein, 1110007C02Rik Protein, AI480648 Protein, As-1 Protein, As-1r Protein, As-1s Protein, As-1t Protein, As1 Protein, As1-r Protein, As1-s Protein, As1-t Protein, Asr-1 Protein, Ast-1 Protein, ARSB Protein, arylsulfatase B Protein, arylsulfatase Protein, arylsulfatase b Protein, ARSB Protein, Arsb Protein, RB348 Protein, LOC5566067 Protein, CpipJ_CPIJ011047 Protein, VDBG_03275 Protein, arsb Protein, LOC5579667 Protein
    Background
    Alternative Names: ASB, G4S, N-acetylgalactosamine-4-sulfatase
    Molecular Weight
    55kDa
    UniProt
    P15848
    Pathways
    Glycosaminoglycan Metabolic Process
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