TUBB Protein (AA 170-419) (His tag)

Catalog No. ABIN6239912

Product Details

Target: TUBB (Tubulin, beta (TUBB))

Biological Activity: Active

Protein Characteristics: AA 170-419

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This TUBB protein is labelled with His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Characteristics: Tag location: N-terminal His Tag

Purity: > 95 %

Biological Activity Comment: Tubulin Beta (TUBb) in molecular biology can refer either to the tubulin protein superfamily of globular proteins, or one of the member proteins of that superfamily. It participate in many essential cellular processes, including mitosis. α- and β-tubulins polymerize into microtubules, a major component of the eukaryotic cytoskeleton. Both α and β tubulins have a mass of around 50kDa and are thus in a similar range compared to actin with ~42kDa. TUBb is one of six members of the tubulin superfamily, which binding drugs to kill cancerous cells by inhibiting microtubule dynamics. Besides, Myxovirus Resistance 1 (MX1) has been identified as an interactor of TUBb, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TUBb and recombinant human MX1. Briefly, TUBb were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to MX1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-TUBb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of TUBb and MX1 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of TUBb with MX1.

Application Details

Application Notes: Isoelectric Point: 6.6

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Target Details

Target: TUBB (Tubulin, beta (TUBB))

Alternative Name: Tubulin Beta (TUBb) (TUBB Products)

Synonyms: M40 Protein, OK/SW-cl.56 Protein, TUBB1 Protein, TUBB5 Protein, XLOT Protein, m40 Protein, ok/sw-cl.56 Protein, tubb1 Protein, tubb5 Protein, TUBB2A Protein, TUBB2B Protein, TUBB Protein, 1t Protein, B1t Protein, BETA 56D Protein, CG9277 Protein, DTB2 Protein, Dmbeta1 Protein, Dmel\\CG9277 Protein, T Protein, Tub Protein, Tubulin Protein, beta Protein, beta-Tub Protein, beta-Tub56D Protein, beta-tub Protein, beta-tubulin56D Protein, beta1 Protein, beta1-Tubulin Protein, beta1-tub Protein, beta1Tub Protein, beta1t Protein, beta1tub Protein, beta56D Protein, betaTub Protein, betaTub1 Protein, beta[[1]] tubulin Protein, beta[[1]]-tubulin Protein, betatub(56D) Protein, 143391_i_at Protein, 3t Protein, B3t Protein, BETA 60D Protein, CG3401 Protein, D.m.BETA-60D Protein, DTB3 Protein, Dmbeta3 Protein, Dmel\\CG3401 Protein, Tub60D Protein, beta-Tub60D Protein, beta-Tub6D Protein, beta3 Protein, beta3 TU Protein, beta3-Tub Protein, beta3-tubulin Protein, beta3TUB Protein, beta3Tub Protein, beta3t Protein, beta60C Protein, betaTub3 Protein, betaTub60C Protein, beta[[3]] tubulin Protein, beta[[3]]-Tub Protein, beta[[3]]-tubulin Protein, betatub60D Protein, p50 Protein, p50/tubulin Protein, p53 Protein, p53/tubulin Protein, 2t Protein, B2t Protein, BETA 85D Protein, BETA2 Protein, CG9359 Protein, D.m.BETA-85D Protein, DTB4 Protein, Dmbeta2 Protein, Dmel\\CG9359 Protein, beta(2)Tu Protein, beta(2)Tub Protein, beta-Tub85D Protein, beta-tub85D Protein, beta2 Protein, beta2-tub Protein, beta2t Protein, beta2tub Protein, beta85D Protein, betaTub2 Protein, beta[[2]]-tubulin Protein, ms(3)KK[D] Protein, 4t Protein, B4t Protein, BETA 98B Protein, CG4869 Protein, DTB1 Protein, Dmbeta4 Protein, Dmel\\CG4869 Protein, beta-Tub97EF Protein, beta4 Protein, beta4t Protein, beta97F Protein, betaTub4 Protein, betaTub98BC Protein, betaTub98C Protein, Tub1 Protein, bmtub1 Protein, Tub3 Protein, bmtub3 Protein, Tub2 Protein, bmtub2 Protein, Tub4 Protein, bmtub4 Protein, LOC100101153 Protein, tubulin beta class I Protein, putative cobalt-zinc-cadmium outer membrane resistance protein Protein, Tubulin beta chain Protein, tubulin beta-2A chain Protein, beta-Tubulin at 56D Protein, beta-Tubulin at 60D Protein, beta-Tubulin at 85D Protein, beta-Tubulin at 97EF Protein, beta-tubulin Protein, tubulin beta 6 class V Protein, beta tubulin Protein, tubulin beta class I L homeolog Protein, tubulin, beta 5 class I Protein, TUBB Protein, czcC Protein, tbb-6 Protein, tubb Protein, LOC462399 Protein, betaTub56D Protein, betaTub60D Protein, betaTub85D Protein, betaTub97EF Protein, Tub1 Protein, Tub3 Protein, Tub2 Protein, Tub4 Protein, TVAG_525430 Protein, TVAG_062880 Protein, TVAG_456920 Protein, LOC100101153 Protein, TUBB6 Protein, Tb927.1.2330 Protein, Tb927.1.2350 Protein, Tb927.1.2370 Protein, Tb927.1.2390 Protein, tubb.L Protein, Tubb Protein, LOC693049 Protein, LOC443015 Protein, Tubb5 Protein

Background: Alternative Names: TUB-B, TUBB5, TUBB1, M40

Molecular Weight: 32kDa

UniProt: P07437

Pathways: Microtubule Dynamics, M Phase