IL-10 Protein (AA 19-178) (His tag)

Catalog No. ABIN6253266

Product Details

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 19-178

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This IL-10 protein is labelled with His tag.

Purpose: IL-10 (human) (rec.) (His)

Specificity: Human IL-10 (aa 19-178) is fused at the C-terminus to a His-tag.

Characteristics: Protein. Human IL-10 (aa 19-178) is fused at the C-terminus to a His-tag. Source: HEK 293 cells. Endotoxin content: <0.01EU/μg protein (LAL test, Lonza). Lyophilized from 0.2μm-filtered solution in PBS. Purity: >95 % (SDS-PAGE). Interleukin-10 (IL-10) is a cytokine produced by activated Th2 cells, B cells, keratinocytes and monocytes/macrophages. In vitro murine and human IL-10 inhibits cytokine synthesis by Th1 cells, natural killer cells and monocytes/macrophages. Several studies have suggested the potential application of IL-10 as an anti-inflammatory agent in the treatment of septic shock and as an immunosuppressive agent in certain T cell mediated autoimmune diseases.

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.01EU/μg protein (LAL test, Lonza).

Biological Activity Comment: Determined by the dose-dependent co-stimulation (with human IL-4) of MC/9 cells. The ED50 was found to be < 2.0 ng/ml, corresponding to a specific activity of > 5 x 105 units/mg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from 0.2μm-filtered solution in PBS.

Handling Advice: Avoid freeze/thaw cycles. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: IL-10 (IL10 Products)

Synonyms: CSIF Protein, GVHDS Protein, IL-10 Protein, IL10A Protein, TGIF Protein, Il-10 Protein, IL10X Protein, interleukin 10 Protein, IL10 Protein, Il10 Protein

Background:

Alternate Names/Synonyms: Interleukin-10, Cytokine Synthesis Inhibitory Factor, CSIF

Product Description: Interleukin-10 (IL-10) is a cytokine produced by activated Th2 cells, B cells, keratinocytes and monocytes/macrophages. In vitro murine and human IL-10 inhibits cytokine synthesis by Th1 cells, natural killer cells and monocytes/macrophages. Several studies have suggested the potential application of IL-10 as an anti-inflammatory agent in the treatment of septic shock and as an immunosuppressive agent in certain T cell mediated autoimmune diseases.

Molecular Weight: ~18kDa (SDS-PAGE)

NCBI Accession: NP_000563

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints