CRP Protein (AA 19-224) (His tag)

Catalog No. ABIN6253726

Product Details

Target: CRP (C-Reactive Protein (CRP))

Protein Type: Recombinant

Protein Characteristics: AA 19-224

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CRP protein is labelled with His tag.

Sequence: AA 19-224

Characteristics: This protein carries a polyhistidine tag at the C-terminus, and has a calculated MW of 24 kDa. The predicted N-terminus is Phe 17. The reducing (R) protein migrates as 26 kDa in SDS-PAGE. More than 90 % rhCRP exists as active homopentamer in analytical SEC-HPLC assay.

Purity: >95 % as determined by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Grade: HPLC verified

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Tris-NaCl buffer pH 8.0

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).

References

Meyer, Ueland: "Targeted quantification of C-reactive protein and cystatin c and its variants by immuno-MALDI-MS." in: Analytical chemistry, Vol. 86, Issue 12, pp. 5807-14, (2014) (PubMed).

Target Details

Target: CRP (C-Reactive Protein (CRP))

Alternative Name: C-Reactive Protein (CRP Products)

Synonyms: PTX1 Protein, crp Protein, AI255847 Protein, Aa1249 Protein, Ab1-341 Protein, Ab2-196 Protein, Ac1-114 Protein, Ac1262 Protein, Ac2-069 Protein, Ba2-693 Protein, APCS Protein, 0610010I23Rik Protein, AW743261 Protein, C77570 Protein, CRP2 Protein, CRP4 Protein, Crp Protein, ESP1 Protein, Hlp Protein, CRP5.1 Protein, zgc:152809 Protein, C-reactive protein Protein, C-reactive protein, pentraxin-related Protein, c-reactive protein, pentraxin-related Protein, cysteine rich protein 2 Protein, CRP Protein, crp Protein, Crp Protein, Crip2 Protein, LOC776376 Protein

Background: C-reactive protein (CRP) is a member of the pentraxin family of proteins that are characterized by a cyclic pentameric structure. Human CRP gene encodes a 224 amino acids precursor. The mature human CRP protein has 206 amino acids that are noncovalently linked to form the pentameter. Human CRP shares 71 % and 64 % amino acid sequence homology with mouse and rat respectively. CRP, synthesized by hepatocytes, is a major acute phase serum protein in human. IL6, IL1 and glucocorticoids are the major inducer of the CRP gene. The physiological role of CRP is to bind to phosphocholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system. CRP binds to phosphocholine on microbes and damaged cells and enhances phagocytosis by macrophages. Thus, CRP participates in the clearance of necrotic and apoptotic cells. CRP rises up to 50,000-fold in acute inflammation, such as infection. It rises above normal limits within 6 hours, and peaks at 48 hours. Its half-life is constant, and therefore its level is mainly determined by the rate of production. It has been shown that high levels of CRP in humans is associated with an increased risk of cardiovascular diseases.

Molecular Weight: 23.9 kDa

NCBI Accession: NP_000558

Pathways: Carbohydrate Homeostasis