CEACAM5 Protein (AA 35-685) (His tag,AVI tag,Biotin)

Catalog No. ABIN6386445

Product Details

Target: CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5))

Protein Type: Recombinant

Protein Characteristics: AA 35-685

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CEACAM5 protein is labelled with His tag,AVI tag,Biotin.

Sequence: AA 35-685

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Purity: >90 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM Potassium glutamate, pH 7.5

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Target Details

Target: CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5))

Alternative Name: CEACAM-5 (CEACAM5 Products)

Synonyms: CD66e Protein, CEA Protein, CEACAM5 Protein, CEACAM Protein, 1600029H12Rik Protein, Psg30 Protein, carcinoembryonic antigen related cell adhesion molecule 5 Protein, carcinoembryonic antigen Protein, carcinoembryonic antigen-related cell adhesion molecule 5 Protein, carcinoembryonic antigen-related cell adhesion molecule 8 Protein, carcinoembryonic antigen gene family Protein, CEACAM5 Protein, Ceacam5 Protein, LOC100409241 Protein, Cea Protein

Background: Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) is also Carcinoembryonic antigen (CEA), Meconium antigen 100, CD antigen CD66e, CEACAM5 belongs to the immunoglobulin superfamily and CEA family. CEACAM5 contains seven Ig-like (immunoglobulin-like) domains. CEACAM5 is homodimer protein which binding of E.coli Dr adhesins leads to dissociation of the homodimer. CEACAM5 is cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. CEACAM5 is receptor for E.coli Dr adhesins.

Molecular Weight: 75.0 kDa

NCBI Accession: NP_004354