M-CSF/CSF1 Protein (AA 33-255) (His tag,AVI tag,Biotin)
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- Target See all M-CSF/CSF1 (CSF1) Proteins
- M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 33-255
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This M-CSF/CSF1 protein is labelled with His tag,AVI tag,Biotin.
- Sequence
- AA 33-255
- Specificity
- Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- Purity
- >90 % as determined by SDS-PAGE.
- Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
- Top Product
- Discover our top product CSF1 Protein
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- Comment
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Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- PBS, pH 7.4
- Handling Advice
- Please avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
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- Target
- M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))
- Alternative Name
- M-CSF (CSF1 Products)
- Background
- The colony stimulating factor 1 (CSF1), also known as macrophage colony-stimulating factor (M-CSF), is a secreted cytokine which influences hematopoietic stem cells to differentiate into macrophages or other related cell types. Eukaryotic cells also produce M-CSF in order to combat intercellular viral infection. It is one of the three experimentally described colony-stimulating factors. M-CSF binds to the colony stimulating factor 1 receptor. Macrophage colony-stimulating factor has been shown to interact with PIK3R2. M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. Locally produced M-CSF in the vessel wall contributes to the development and progression of atherosclerosis.
- Molecular Weight
- 28.8 kDa
- NCBI Accession
- NP_757349
- Pathways
- RTK Signaling
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