PSMB7 Protein (AA 44-277) (His tag)

Catalog No. ABIN6386905

Product Details

Target: PSMB7 (Proteasome (Prosome, Macropain) Subunit, beta Type, 7 (PSMB7))

Protein Type: Recombinant

Protein Characteristics: AA 44-277

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PSMB7 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Characteristics: PSMB7, 44-277aa, Human, His tag, E.coli

Purity: > 80 % by SDS - PAGE

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/ml (determined by Bradford assay)

Buffer: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT

Storage: 4 °C

Target Details

Target: PSMB7 (Proteasome (Prosome, Macropain) Subunit, beta Type, 7 (PSMB7))

Alternative Name: PSMB7 (PSMB7 Products)

Synonyms: DDBDRAFT_0185624 Protein, DDBDRAFT_0232933 Protein, DDB_0185624 Protein, DDB_0232933 Protein, AU020723 Protein, MC14 Protein, Z Protein, 20S proteasome subunit beta-7 Protein, proteasome (prosome, macropain) subunit, beta type, 7 S homeolog Protein, proteasome subunit beta 7 Protein, proteasome (prosome, macropain) subunit, beta type, 7 Protein, proteasome (prosome, macropain) subunit, beta type 7 Protein, psmB7 Protein, psmb7.S Protein, PSMB7 Protein, Psmb7 Protein

Background: PSMB7, also known as proteasome subunit beta type-7, is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family that is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon and proteolytic processing is required to generate a mature subunit. This subunit is not present in the immunoproteasome and is replaced by catalytic subunit 2i. Recombinant human PSMB7 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. Synonyms: Macropain chain Z, Multicatalytic endopeptidase complex chain Z, Proteasome subunit Z, Proteasome subunit beta type 7. NCBI no.: NP_002790

Molecular Weight: 27.6 kDa (255aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA