Beclin 1 Protein (His tag)
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- Target See all Beclin 1 (BECN1) Proteins
- Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Beclin 1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product BECN1 Protein
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Beclin 1, Autophagy Related (BECN1) (AA 1-450) protein (GST tag)
BECN1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Beclin 1, Autophagy Related (BECN1) (AA 46-152) protein (His-IF2DI Tag)BECN1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Beclin 1, Autophagy Related (BECN1) (AA 351-450) protein (GST tag)BECN1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Beclin 1, Autophagy Related (BECN1) (AA 1-450) protein (His tag)BECN1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Beclin 1, Autophagy Related (BECN1) (AA 150-450) protein (His tag)Crystallography grade BECN1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Beclin 1, Autophagy Related (BECN1) (AA 134-450) protein (His tag)Crystallography grade BECN1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Beclin 1, Autophagy Related (BECN1) (AA 132-448) protein (His tag)Crystallography grade BECN1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Beclin 1, Autophagy Related (BECN1) (AA 148-448) protein (His tag)Crystallography grade BECN1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Beclin 1, Autophagy Related (BECN1) (AA 1-450) protein (Strep Tag)Crystallography grade BECN1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Beclin 1, Autophagy Related (BECN1) (AA 1-448) protein (Strep Tag)Crystallography grade BECN1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- Comment
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Synonyms: beclin 1, ATG6, beclin1, VPS30
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.5) containing 0.1 M NaCl, 10% glycerol,1 MM DTT
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))
- Alternative Name
- BECN1 (BECN1 Products)
- Synonyms
- beclin-1 Protein, atg6 Protein, vps30 Protein, beclin1 Protein, Beclin-1 Protein, becn1 Protein, zgc:73097 Protein, ATG6 Protein, VPS30 Protein, 4921513J16Rik Protein, 5430417M23Rik Protein, Atg6 Protein, beclin 1 Protein, beclin 1, autophagy related Protein, beclin 1 protein Protein, beclin-1 Protein, beclin family protein Protein, beclin 1, autophagy related L homeolog Protein, BECN1 Protein, becn1 Protein, atg6 Protein, CpipJ_CPIJ011087 Protein, PTRG_07825 Protein, SJAG_01876 Protein, Becn1 Protein, becn1.L Protein
- Background
- BECN1, also as known as Beclin-1 and autophagy-related gene (Atg) 6, belongs to the beclin family. This protein plays a central role in autophagy. Beclin-1 and its binding partner class III phosphoinositide 3-kinase (PI3K), also named Vps34, are required for the initiation of the formation of the autophagasome in autophagy. Also it required for the abcission step of cytokinesis. BECN1 plays a role in antiviral host defense. Recombinant human BECN1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
- Molecular Weight
- 54.1 kDa (470aa)
- NCBI Accession
- NP_003757
- Pathways
- Autophagy
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