GATC Protein (AA 1-136) (His tag)
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- Target See all GATC Proteins
- GATC (Glutamyl-tRNA Amidotransferase, Subunit C (GATC))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-136
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This GATC protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMWSRLVW LGLRAPLGGR QGFTSKADPQ GSGRITAAVI EHLERLALVD FGSREAVARL EKAIAFADRL RAVDTDGVEP MESVLEDRCL YLRSDNVVEG NCADELLQNS HRVVEEYFVA PPGNISLPKL DEQEPFPHS
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product GATC Protein
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Glutamyl-tRNA Amidotransferase, Subunit C (GATC) (AA 1-136) protein (His tag)
GATC Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Glutamyl-tRNA Amidotransferase, Subunit C (GATC) (AA 1-136) protein (GST tag)GATC Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Glutamyl-tRNA Amidotransferase, Subunit C (GATC) (AA 21-155) protein (His tag)Crystallography grade GATC Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Glutamyl-tRNA Amidotransferase, Subunit C (GATC) protein (Myc-DYKDDDDK Tag)GATC Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 30 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- GATC (Glutamyl-tRNA Amidotransferase, Subunit C (GATC))
- Alternative Name
- GATC (GATC Products)
- Synonyms
- 15E1.2 Protein, RGD1309698 Protein, 2010003O18Rik Protein, gatc Protein, glutamyl-tRNA amidotransferase subunit C Protein, glutamyl-tRNA(Gln) amidotransferase, subunit C Protein, si:rp71-1c23.3 Protein, GATC Protein, Gatc Protein, gatc Protein, si:rp71-1c23.3 Protein
- Background
- GATC allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). This protein is subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A (QRSL1), B (PET112) and C (GATC) subunits. Recombinant human GATC protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 17.5 kDa (159aa), confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
- NCBI Accession
- NP_789788
- UniProt
- O43716
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