VAV1 Protein (AA 189-565) (His tag)
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- Target See all VAV1 Proteins
- VAV1 (Vav 1 Oncogene (VAV1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 189-565
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This VAV1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMTEYDKR CCCLREIQQT EEKYTDTLGS IQQHFLKPLQ RFLKPQDIEI IFINIEDLLR VHTHFLKEMK EALGTPGAAN LYQVFIKYKE RFLVYGRYCS QVESASKHLD RVAAAREDVQ MKLEECSQRA NNGRFTLRDL LMVPMQRVLK YHLLLQELVK HTQEAMEKEN LRLALDAMRD LAQCVNEVKR DNETLRQITN FQLSIENLDQ SLAHYGRPKI DGELKITSVE RRSKMDRYAF LLDKALLICK RRGDSYDLKD FVNLHSFQVR DDSSGDRDNK KWSHMFLLIE DQGAQGYELF FKTRELKKKW MEQFEMAISN IYPENATANG HDFQMFSFEE TTSCKACQML LRGTFYQGYR CHRCRASAHK ECLGRVPPCG
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product VAV1 Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.2M NaCl, 30 % glycerol, 2 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- VAV1 (Vav 1 Oncogene (VAV1))
- Alternative Name
- VAV1 (VAV1 Products)
- Synonyms
- VAV Protein, Vav Protein, vav-T Protein, vav guanine nucleotide exchange factor 1 Protein, vav 1 oncogene Protein, vav guanine nucleotide exchange factor 1 L homeolog Protein, VAV1 Protein, Vav1 Protein, vav1.L Protein
- Background
- Proto-oncogene vav isoform 1, also known as VAV1, is a member of the VAV gene family. The VAV1 proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. In addition, VAV1 contains an SH2 domain, which could indicate its role as a substrate for tyrosine kinases. Expression of VAV1 is limited exclusively to cells of hematopoietic origin, including those of the erythroid, lymphoid and myeloid lineages. Recombinant human VAV1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 46.8 kDa (400aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_005419
- UniProt
- P15498
- Pathways
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, BCR Signaling
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