SEC61B Protein (AA 1-70) (His tag)
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- Target See all SEC61B Proteins
- SEC61B (Sec61 beta Subunit (SEC61B))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-70
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This SEC61B protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMPGPTPS GTNVGSSGRS PSKAVAARAA GSTVRQRKNA SCGTRSAGRT TSAGTGGMWR FYTEDSPGLK VGP
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product SEC61B Protein
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Sec61 beta Subunit (SEC61B) (AA 1-70) protein (His tag)
SEC61B Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Sec61 beta Subunit (SEC61B) (AA 1-66) protein (GST tag)SEC61B Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Sec61 beta Subunit (SEC61B) (AA 1-96) protein (GST tag)SEC61B Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Sec61 beta Subunit (SEC61B) (AA 2-96) protein (rho-1D4 tag)Crystallography grade SEC61B Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Sec61 beta Subunit (SEC61B) (AA 2-96) protein (rho-1D4 tag)Crystallography grade SEC61B Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.2M NaCl, 50 % glycerol, 2 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- SEC61B (Sec61 beta Subunit (SEC61B))
- Alternative Name
- SEC61B (SEC61B Products)
- Synonyms
- 1190006C12Rik Protein, AI326121 Protein, AW122942 Protein, Sec61 translocon beta subunit Protein, Sec61 beta subunit Protein, SEC61B Protein, Sec61b Protein
- Background
- The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript. Recombinant human SEC61B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 9.4kDa (93aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_006799
- UniProt
- P60468
- Pathways
- Protein targeting to Nucleus
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