PDIA6 Protein (AA 20-440) (His tag)
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- Target See all PDIA6 Proteins
- PDIA6 (Protein Disulfide Isomerase Family A, Member 6 (PDIA6))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 20-440
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PDIA6 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MLYSSSDDVI ELTPSNFNRE VIQSDSLWLV EFYAPWCGHC QRLTPEWKKA ATALKDVVKV GAVDADKHHS LGGQYGVQGF PTIKIFGSNK NRPEDYQGGR TGEAIVDAAL SALRQLVKDR LGGRSGGYSS GKQGRSDSSS KKDVIELTDD SFDKNVLDSE DVWMVEFYAP WCGHCKNLEP EWAAAASEVK EQTKGKVKLA AVDATVNQVL ASRYGIRGFP TIKIFQKGES PVDYDGGRTR SDIVSRALDL FSDNAPPPEL LEIINEDIAK RTCEEHQLCV VAVLPHILDT GAAGRNSYLE VLLKLADKYK KKMWGWLWTE AGAQSELETA LGIGGFGYPA MAAINARKMK FALLKGSFSE QGINEFLREL SFGRGSTAPV GGGAFPTIVE REPWDGRDGE LPVEDDIDLS DVELDDLGKD EL
- Purity
- > 90 % by SDS - PAGE
- Biological Activity Comment
- Specific activity > 20 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT.
- Top Product
- Discover our top product PDIA6 Protein
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Protein Disulfide Isomerase Family A, Member 6 (PDIA6) (AA 1-440) protein (GST tag)
PDIA6 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Protein Disulfide Isomerase Family A, Member 6 (PDIA6) (AA 20-440) protein (His tag)Crystallography grade PDIA6 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Protein Disulfide Isomerase Family A, Member 6 (PDIA6) (AA 20-440) protein (His tag)Crystallography grade PDIA6 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Protein Disulfide Isomerase Family A, Member 6 (PDIA6) protein (Myc-DYKDDDDK Tag)PDIA6 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing, 50 mM NaCl, 2 mM DTT and 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- PDIA6 (Protein Disulfide Isomerase Family A, Member 6 (PDIA6))
- Alternative Name
- PDIA6 (PDIA6 Products)
- Synonyms
- 1700015E05Rik Protein, AL023058 Protein, C77895 Protein, CaBP5 Protein, P5 Protein, Txndc7 Protein, ERP5 Protein, TXNDC7 Protein, CaBP1 Protein, pdi-p5 Protein, erp5 Protein, pdia6 Protein, pdip5 Protein, txndc7 Protein, protein disulfide isomerase family A member 6 Protein, protein disulfide isomerase associated 6 Protein, protein disulfide isomerase family A, member 6 Protein, protein disulfide isomerase family A member 6 L homeolog Protein, PDIA6 Protein, Pdia6 Protein, pdia6.L Protein
- Background
- PDIA6 is a member of the protein disulfide isomerase (PDI). PDI is an enzyme in the endoplasmic reticulum in eukaryotes or periplasmic space of prokaryotes that catalyzes the formation and breakage of disulfide bonds between cysteine residues within proteins as they fold. PDIA6 function as a chaperone that inhibits aggregation of misfolded proteins. It plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin. Recombinant human PDIA6 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 48.5 kDa (442aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_005733
- UniProt
- Q15084
- Pathways
- ER-Nucleus Signaling, Cell RedoxHomeostasis
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