GSTM5 Protein (AA 1-218) (His tag)
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- Target See all GSTM5 Proteins
- GSTM5 (Glutathione S-Transferase mu 5 (GSTM5))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-218
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This GSTM5 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSHMPMTLG YWDIRGLAHA IRLLLEYTDS SYVEKKYTLG DAPDYDRSQW LNEKFKLGLD FPNLPYLIDG AHKITQSNAI LRYIARKHNL CGETEEEKIR VDILENQVMD NHMELVRLCY DPDFEKLKPK YLEELPEKLK LYSEFLGKRP WFAGDKITFV DFLAYDVLDM KRIFEPKCLD AFLNLKDFIS RFEGLKKISA YMKSSQFLRG LLFGKSATWN SK
- Purity
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 90,000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.
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Glutathione S-Transferase mu 5 (GSTM5) (AA 145-218) protein (GST tag)
GSTM5 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Glutathione S-Transferase mu 5 (GSTM5) (AA 1-218) protein (His tag)GSTM5 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Glutathione S-Transferase mu 5 (GSTM5) (AA 1-218) protein (GST tag)GSTM5 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Glutathione S-Transferase mu 5 (GSTM5) (AA 1-224) protein (Strep Tag)Crystallography grade GSTM5 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Glutathione S-Transferase mu 5 (GSTM5) protein (Myc-DYKDDDDK Tag)GSTM5 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol, 0.1M NaCl
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- GSTM5 (Glutathione S-Transferase mu 5 (GSTM5))
- Alternative Name
- GSTM5 (GSTM5 Products)
- Synonyms
- GSTM5-5 Protein, GTM5 Protein, glutathione S-transferase mu 5 Protein, glutathione S-transferase, mu 5 Protein, GSTM5 Protein, Gstm5 Protein
- Background
- Glutathione S-transferase mu 5, also known as GSTM5, is member of the glutathione s-transferase (GST) family of proteins. There are eight families of GST proteins, namely alpha, kappa, mu, omega, pi, sigma, theta and zeta, each of which is composed of proteins that have a variety of functions throughout the cell. GSTM5 plays an important role in detoxification. Recombinant human GSTM5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 28.2kDa (242aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_000842
- UniProt
- P46439
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