PGD Protein (AA 1-483) (His tag)

Catalog No. ABIN6387696

Product Details

Target: PGD (Phosphogluconate Dehydrogenase (PGD))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 1-483

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PGD protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSH MAQADIALIG LAVMGQNLIL NMNDHGFVVC AFNRTVSKVD DFLANEAKGT KVVGAQSLKE MVSKLKKPRR IILLVKAGQA VDDFIEKLVP LLDTGDIIID GGNSEYRDTT RRCRDLKAKG ILFVGSGVSG GEEGARYGPS LMPGGNKEAW PHIKTIFQGI AAKVGTGEPC CDWVGDEGAG HFVKMVHNGI EYGDMQLICE AYHLMKDVLG MAQDEMAQAF EDWNKTELDS FLIEITANIL KFQDTDGKHL LPKIRDSAGQ KGTGKWTAIS ALEYGVPVTL IGEAVFARCL SSLKDERIQA SKKLKGPQKF QFDGDKKSFL EDIRKALYAS KIISYAQGFM LLRQAATEFG WTLNYGGIAL MWRGGCIIRS VFLGKIKDAF DRNPELQNLL LDDFFKSAVE NCQDSWRRAV STGVQAGIPM PCFTTALSFY DGYRHEMLPA SLIQAQRDYF GAHTYELLAK PGQFIHTNWT GHGGTVSSSS YNA

Purity: > 90 % by SDS - PAGE

Biological Activity Comment: Specific activity is > 10 units/mg, in which one unit oxidize 1.0 umole of 6-phospho-D-gluconate to D-ribulose 5-phosphate per minute at pH 8.0 at 25C, in the presence of beta-NADP.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Bioactivity Validated

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In 20 mM Tris-HCl buffer (  pH 8.0) containing 1  mM DTT, 0.1M Nacl, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: PGD (Phosphogluconate Dehydrogenase (PGD))

Alternative Name: PGD (PGD Products)

Synonyms: 6PGD Protein, 6-PGD Protein, 6-Pgd Protein, 6-pgd Protein, 6PGDH Protein, 6Pgd Protein, 6Pgdh Protein, 6pgdh Protein, A7 Protein, CG3724 Protein, Dmel\\CG3724 Protein, EG:87B1.4 Protein, N1 Protein, PGD Protein, Pdg Protein, l(1)2Dc Protein, l(1)2De Protein, l(1)A7 Protein, l(1)G0385 Protein, l(1)N1 Protein, l(1)N3[90] Protein, l(1)Pgd Protein, l(1)Pgd-A Protein, l35 Protein, pgd Protein, 0610042A05Rik Protein, AU019875 Protein, C78335 Protein, fi03d03 Protein, zgc:55585 Protein, wu:fi03d03 Protein, zgc:111848 Protein, 6-PGDH Protein, GND1b Protein, Pgd Protein, Cc2-27 Protein, phosphogluconate dehydrogenase Protein, Phosphogluconate dehydrogenase Protein, phosphogluconate dehydrogenase L homeolog Protein, 6-phosphogluconate dehydrogenase, decarboxylating Protein, 6-phosphogluconate dehydrogenase, decarboxylating-like Protein, PGD Protein, Pgd Protein, pgd.L Protein, pgd Protein, GND1a|GND1b Protein, Thal_1580 Protein, GY4MC1_3101 Protein, Odosp_2189 Protein, Marky_1024 Protein, gnd Protein, LOC101451876 Protein

Background: PGD (Phosphogluconate dehydrogenas), also known as 6PGD, is a 483 amino acid enzyme that is involved in the pentose phosphate shunt. Pentose is required for nucleic acid biosynthesis and the pentose phosphate cycle is a major source of NADPH. PGD deficiency increases the level of erythrocyte pyruvate kinase (PK) activity and reduces glutathione synthetase (GSH), resulting in hemolysis. Defects in PGD are generally asymptomatic and are inherited in an autosomal dominant fashion. Recombinant human PGD protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 55.3 kDa (503aa)

NCBI Accession: NP_002622

UniProt: P52209