NME4 Protein (AA 33-187) (His tag)
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- Target See all NME4 Proteins
- NME4 (NME/NM23 Nucleoside Diphosphate Kinase 4 (NME4))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 33-187
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NME4 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MPSWTRERTL VAVKPDGVQR RLVGDVIQRF ERRGFTLVGM KMLQAPESVL AEHYQDLRRK PFYPALIRYM SSGPVVAMVW EGYNVVRASR AMIGHTDSAE AAPGTIRGDF SVHISRNVIH ASDSVEGAQR EIQLWFQSSE LVSWADGGQH SSIHPA
- Purity
- > 90 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 120 units/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.
- Top Product
- Discover our top product NME4 Protein
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NME/NM23 Nucleoside Diphosphate Kinase 4 (NME4) (AA 33-187) protein (His tag)
NME4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
NME/NM23 Nucleoside Diphosphate Kinase 4 (NME4) (AA 99-187) protein (GST tag)NME4 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
NME/NM23 Nucleoside Diphosphate Kinase 4 (NME4) (AA 1-187) protein (GST tag)NME4 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
NME/NM23 Nucleoside Diphosphate Kinase 4 (NME4) (AA 34-187) protein (His tag)Crystallography grade NME4 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
NME/NM23 Nucleoside Diphosphate Kinase 4 (NME4) (AA 33-186) protein (His tag)Crystallography grade NME4 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
NME/NM23 Nucleoside Diphosphate Kinase 4 (NME4) protein (Myc-DYKDDDDK Tag)NME4 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. 20 mM Tris-HCl buffer ( pH 8.0) containing 40 % glycerol, 0.2M NaCl
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- NME4 (NME/NM23 Nucleoside Diphosphate Kinase 4 (NME4))
- Alternative Name
- NME4 (NME4 Products)
- Synonyms
- NDPK-D Protein, NM23H4 Protein, nm23-H4 Protein, 2610027N22Rik Protein, 2810024O08Rik Protein, 5730493H09Rik Protein, NM23-M4 Protein, Nm23M4 Protein, NME/NM23 nucleoside diphosphate kinase 4 Protein, NME4 Protein, Nme4 Protein
- Background
- NME4, also known as nucleoside diphosphate kinase, mitochondrial, belongs to the NDK family. NME4 are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4. NME4 plays a major role in the synthesis of nucleoside triphosphates other than ATP. Recombinant human NME4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
- Molecular Weight
- 19.6 kDa (176aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_005000
- UniProt
- O00746
- Pathways
- Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process
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