SPOCK3 Protein (AA 22-436) (His tag)
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- Target See all SPOCK3 Proteins
- SPOCK3 (Sparc/osteonectin, Cwcv and Kazal-Like Domains Proteoglycan (Testican) 3 (SPOCK3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 22-436
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Origin
- Mouse
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This SPOCK3 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- AAAAVAVAGG RSDGGNFLDE KQWLTTISQY DKEVGQWNKF RDEVEDDYFR TWNPGKPFDQ ALDPAKDPCL KTKCSRHKVC ITQDAQTALC ISHRRLTHSM KEVGGSHKQW RGLPSSTCKP CPIAYASPVC GSDGHSYSSQ CKLEYQACVL GKQISIKCEG RCPCPSDKSM NIGRNVKRAC SDLEFREVAN RLRDWFKALH ESGSQNKKTK ALLRPERSRF DTSILPICKD SLGWMFNRLD TNYDLLLDQS ELGSIYLDKN EQCTKAFFNS CDTYKDSLIS NNEWCYCFQR QQDPPCHTEL SNIQKRQGIK KLLGQYIPLC DEDGYYKPTQ CHGSVGQCWC VDRYGNEVVG SRINGVADCA IDFEISGDFA SGDFREWTDD EGEEDDIMND KDDIEDDDED EGDDDDDGDV HDGYILEHHH HHH
- Purity
- > 90 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Top Product
- Discover our top product SPOCK3 Protein
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Sparc/osteonectin, Cwcv and Kazal-Like Domains Proteoglycan (Testican) 3 (SPOCK3) protein (His tag)
SPOCK3 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Sparc/osteonectin, Cwcv and Kazal-Like Domains Proteoglycan (Testican) 3 (SPOCK3) (AA 1-433) protein (GST tag)SPOCK3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Sparc/osteonectin, Cwcv and Kazal-Like Domains Proteoglycan (Testican) 3 (SPOCK3) (AA 132-230) protein (GST tag)SPOCK3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Sparc/osteonectin, Cwcv and Kazal-Like Domains Proteoglycan (Testican) 3 (SPOCK3) (AA 22-436) protein (His tag)Crystallography grade SPOCK3 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Sparc/osteonectin, Cwcv and Kazal-Like Domains Proteoglycan (Testican) 3 (SPOCK3) (AA 23-436) protein (His tag)Crystallography grade SPOCK3 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- SPOCK3 (Sparc/osteonectin, Cwcv and Kazal-Like Domains Proteoglycan (Testican) 3 (SPOCK3))
- Alternative Name
- Spock3 (SPOCK3 Products)
- Synonyms
- im:7139568 Protein, si:dkey-285b22.1 Protein, HSAJ1454 Protein, TES-3 Protein, TICN3 Protein, Testican-3 Protein, 2900045C01Rik Protein, AI428471 Protein, mKIAA4039 Protein, sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 3 Protein, SPARC/osteonectin, cwcv and kazal like domains proteoglycan 3 Protein, sparc/osteonectin, cwcv and kazal-like domains proteoglycan 3 Protein, spock3 Protein, SPOCK3 Protein, Spock3 Protein
- Background
- Spock3, also known as testican-3, is a nervous system-expressed heparan sulfate proteoglycan belonging to a subgroup of the BM-40/SPARC/osteonectin family, the role of which in brain development is unclear. It inhibits the processing of pro-matrix metalloproteinase 2(MMP-2) by MT1-MMP and MT3-MMP. It is mostly confined to the developmental stage of the brain. Recombinant mouse Spock3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 47.9kDa (423aa) 40-57kDa (SDS-PAGE under reducing conditions)
- NCBI Accession
- NP_001239549
- UniProt
- Q8BKV0
- Pathways
- Glycosaminoglycan Metabolic Process
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