Renin Protein (REN) (AA 24-406) (His tag)
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- Target See all Renin (REN) Proteins
- Renin (REN)
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 24-406
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Origin
- Mouse
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This Renin protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMEPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERAKFLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGKTSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA LEHHHHHH
- Purity
- > 90 % by SDS - PAGE.
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 9,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of ubiquitin-AMC per minute at pH 7.5, at 37C.
- Top Product
- Discover our top product REN Protein
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Renin (REN) protein (His tag)
REN Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Renin (REN) (AA 24-406) protein (GST tag)REN Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Renin (REN) (Active) protein (His tag)REN Origin: Mouse Host: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
Renin (REN) (AA 67-406) protein (His tag)Crystallography grade REN Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Renin (REN) (AA 72-402) protein (His tag)Crystallography grade REN Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- Renin (REN)
- Alternative Name
- REN (REN Products)
- Synonyms
- HNFJ2 Protein, RATRENAA Protein, RENAA Protein, Ren1 Protein, D19352 Protein, Ren Protein, Ren-1 Protein, Ren-A Protein, Ren1c Protein, Ren1d Protein, Rn-1 Protein, Rnr Protein, renin Protein, renin 1 structural Protein, REN Protein, Ren Protein, Ren1 Protein, ren Protein
- Background
- Uchl3, as known as ubiquitin carboxyl-terminal hydrolase isozyme L3, is a member of the peptidase C12 family of deubiquitinating enzymes. It is widely expressed with the highest levels being observed in heart, testis, thymus and striated muscle. This protein is suggested to function in the central nervous system. In particular, it is involved in the maintenance of neurons of the gracile tract, nucleus tractus, solitaris, and area postrema, and in working memory. Recombinant mouse Uchl3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 27.2 kDa (238aa) 28-40kDa (SDS-PAGE under reducing conditions)
- NCBI Accession
- NP_057932
- UniProt
- Q9JKB1
- Pathways
- ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour
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