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PGAM2 Protein (AA 1-254) (His tag)

PGAM2 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS Active
Catalog No. ABIN6387878
  • Target See all PGAM2 Proteins
    PGAM2 (phosphoglycerate Mutase 2 (Muscle) (PGAM2))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Protein Characteristics
    AA 1-254
    Origin
    • 5
    • 1
    • 1
    • 1
    Human
    Source
    • 4
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This PGAM2 protein is labelled with His tag.
    Application
    SDS-PAGE (SDS)
    Sequence
    MGSSHHHHHH SSGLVPRGSH MATHRLVMVR HGESTWNQEN RFCGWFDAEL SEKGTEEAKR GAKAIKDAKM EFDICYTSVL KRAIRTLWAI LDGTDQMWLP VVRTWRLNER HYGGLTGLNK AETAAKHGEE QVKIWRRSFD IPPPPMDEKH PYYNSISKER RYAGLKPGEL PTCESLKDTI ARALPFWNEE IVPQIKAGKR VLIAAHGNSL RGIVKHLEGM SDQAIMELNL PTGIPIVYEL NKELKPTKPM QFLGDEETVR KAMEAVAAQG KAK
    Purity
    > 95 % by SDS - PAGE
    Biological Activity Comment
    Specific activity is >100units/mg, in which One unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37C.
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    Discover our top product PGAM2 Protein
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Bioactivity Validated

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Liquid. 20  mM Tris-HCl buffer (  pH 8.0) containing 20 % glycerol, 0.1M NaCl, 1  mM DTT
    Storage
    4 °C,-20 °C,-80 °C
    Storage Comment
    Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
  • Target
    PGAM2 (phosphoglycerate Mutase 2 (Muscle) (PGAM2))
    Alternative Name
    PGAM2 (PGAM2 Products)
    Synonyms
    zgc:63876 Protein, GSD10 Protein, PGAM-M Protein, PGAMM Protein, D14Mgh1 Protein, Pgmut Protein, phosphoglycerate mutase 2 Protein, phosphoglycerate mutase 2 (muscle) Protein, phosphoglycerate mutase 2 L homeolog Protein, Pgam2 Protein, PGAM2 Protein, pgam2 Protein, pgam2.L Protein
    Background
    PGAM2, also known as phosphoglycerate mutase 2, belongs to the phosphoglycerate mutase family. Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). This gene encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase efficiency, also known as glycogen storage disease X. Recombinant human PGAM2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
    Molecular Weight
    30.9 kDa (273aa) confirmed by MALDI-TOF
    NCBI Accession
    NP_000281
    UniProt
    P15259
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