TPI1 Protein (AA 1-249) (His tag)

Catalog No. ABIN6387938

Product Details

Target: TPI1 (Triosephosphate Isomerase 1 (TPI1))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 1-249

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This TPI1 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSH MAPSRKFFVG GNWKMNGRKQ SLGELIGTLN AAKVPADTEV VCAPPTAYID FARQKLDPKI AVAAQNCYKV TNGAFTGEIS PGMIKDCGAT WVVLGHSERR HVFGESDELI GQKVAHALAE GLGVIACIGE KLDEREAGIT EKVVFEQTKV IADNVKDWSK VVLAYEPVWA IGTGKTATPQ QAQEVHEKLR GWLKSNVSDA VAQSTRIIYG GSVTGATCKE LASQPDVDGF LVGGASLKPE FVDIINAKQ

Purity: > 95 % by SDS - PAGE

Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 3000 units/mg, in which one unit will convert 1.0 umole of D-glyceraldehyde-3-phosphate to dihydroxyacetone phosphate per minute at pH 7.5 at 25C.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Bioactivity Validated

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 10 % glycerol, 1  mM DTT.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: TPI1 (Triosephosphate Isomerase 1 (TPI1))

Alternative Name: TPI1 (TPI1 Products)

Synonyms: TIM Protein, TPI Protein, TIM1 Protein, hTIM Protein, tpi Protein, tpi1 Protein, TPI1 Protein, AI255506 Protein, Tpi Protein, Tpi-1 Protein, GB17473 Protein, Tim Protein, CG2171 Protein, Dmel\\CG2171 Protein, ND14 Protein, TPIS Protein, sgk Protein, wstd Protein, DrTPI-A Protein, DrTPI-B Protein, TIM-B Protein, cb179 Protein, wu:fb51c02 Protein, triosephosphate isomerase 1 Protein, timeless circadian regulator Protein, triosephosphate isomerase 1 S homeolog Protein, triosephosphate isomerase Protein, triose-phosphate isomerase Protein, Triose-phosphate isomerase Protein, triose-phosphate isomerase, putative Protein, timeless circadian clock Protein, Triose phosphate isomerase Protein, Triosephosphate isomerase Protein, triosephosphate isomerase 1a Protein, triosephosphate isomerase 1b Protein, TPI1 Protein, TIMELESS Protein, tpi1 Protein, Tpi1 Protein, tpi1.S Protein, Tpi Protein, tpiA Protein, CNE05000 Protein, Fnod_0738 Protein, Tlet_0996 Protein, SARE_RS16215 Protein, PKH_126640 Protein, Timeless Protein, tpi-1 Protein, tpi1a Protein, tpi1b Protein

Background: TPI1 (Triosephosphate isomerase) belongs to the triosephosphate isomerase family. TPI1 catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection. Recombinant human TPI1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 28.8kDa (269aa) confirmed by MALDI-TOF

NCBI Accession: NP_000356

UniProt: P60174

Pathways: Cell RedoxHomeostasis