ENO2/NSE Protein (AA 1-434) (His tag)
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- Target See all ENO2/NSE (ENO2) Proteins
- ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-434
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This ENO2/NSE protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMSIEKIW AREILDSRGN PTVEVDLYTA KGLFRAAVPS GASTGIYEAL ELRDGDKQRY LGKGVLKAVD HINSRIAPAL ISSGISVVEQ EKLDNLMLEL DGTENKSKFG ANAILGVSLA VCKAGAAERD LPLYRHIAQL AGNSDLILPV PAFNVINGGS HAGNKLAMQE FMILPVGAES FRDAMRLGAE VYHTLKGVIK DKYGKDATNV GDEGGFAPNI LENSEALELV KEAIDKAGYT EKMVIGMDVA ASEFYRDGKY DLDFKSPADP SRYITGDQLG ALYQDFVRNY PVVSIEDPFD QDDWAAWSKF TANVGIQIVG DDLTVTNPKR IERAVEEKAC NCLLLKVNQI GSVTEAIQAC KLAQENGWGV MVSHRSGETE DTFIADLVVG LCTGQIKTGA PCRSERLAKY NQLMRIEEEL GDEARFAGHN FRNPSVL
- Purity
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 10,000 pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37C.
- Top Product
- Discover our top product ENO2 Protein
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Enolase 2 (Gamma, Neuronal) (ENO2) (AA 1-434) protein (GST tag)
ENO2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Enolase 2 (Gamma, Neuronal) (ENO2) (AA 325-434) protein (GST tag)ENO2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Enolase 2 (Gamma, Neuronal) (ENO2) (AA 1-434) protein (His tag)ENO2 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Enolase 2 (Gamma, Neuronal) (ENO2) (AA 1-434) proteinENO2 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
Enolase 2 (Gamma, Neuronal) (ENO2) (AA 2-434) protein (His tag)Crystallography grade ENO2 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Enolase 2 (Gamma, Neuronal) (ENO2) (AA 2-434) protein (His tag)Crystallography grade ENO2 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))
- Alternative Name
- Eno2 (ENO2 Products)
- Synonyms
- ENO2 Protein, DKFZp459B1817 Protein, NSE Protein, AI837106 Protein, D6Ertd375e Protein, Eno-2 Protein, RNEN3 Protein, eno3 Protein, wu:fc09h05 Protein, zgc:92418 Protein, enolase 2 Protein, enolase 2 (gamma, neuronal) Protein, enolase 2, gamma neuronal Protein, enolase 2, gamma, neuronal Protein, ENO2 Protein, Eno2 Protein, eno2 Protein
- Background
- Eno2, also known as Gamma enolase isoform1, is a glycolytic isoenzyme which is located in central and peripheral neurons and neuroendocrine cells. This enzyme is released into the CSF when neural tissue is injured. Neoplasms derived from neural or neuroendocrine tissue may release Eno2 into the blood. Eno2 is a useful substance that has been detected in patients with certain tumors, namely: neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma. Recombinant mouse Eno2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 49.7kDa (457aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_038537
- UniProt
- P17183
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