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PHPT1 Protein (AA 1-125) (His tag)

PHPT1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS Active
Catalog No. ABIN6388004
  • Target See all PHPT1 Proteins
    PHPT1 (phosphohistidine Phosphatase 1 (PHPT1))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Protein Characteristics
    AA 1-125
    Origin
    • 8
    • 2
    • 1
    • 1
    • 1
    Human
    Source
    • 7
    • 4
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This PHPT1 protein is labelled with His tag.
    Application
    SDS-PAGE (SDS)
    Sequence
    MGSSHHHHHH SSGLVPRGSH MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY
    Purity
    > 95 % by SDS - PAGE
    Biological Activity Comment
    Specific activity is >120 units/mg, and is defined as the amount of enzyme that hydrolyze 1nmol f p-nitrophenyl phosphate per minute at pH7.5 at 37C.
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  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Bioactivity Validated

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Liquid. In 20 mM Tris-HCl buffer (  pH 8.0) containing 0.2M NaCl, 2  mM DTT, 10 % glycerol
    Storage
    4 °C,-20 °C,-80 °C
    Storage Comment
    Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
  • Target
    PHPT1 (phosphohistidine Phosphatase 1 (PHPT1))
    Alternative Name
    PHPT1 (PHPT1 Products)
    Synonyms
    wu:fb50e08 Protein, zgc:101086 Protein, MGC131298 Protein, PHP14 Protein, CGI-202 Protein, HSPC141 Protein, RP11-216L13.10 Protein, bA216L13.10 Protein, 1700008C22Rik Protein, Php14 Protein, phosphohistidine phosphatase 1 Protein, phosphohistidine phosphatase 1 L homeolog Protein, 14 kDa phosphohistidine phosphatase-like Protein, LOC424401 Protein, phpt1 Protein, PHPT1 Protein, phpt1.L Protein, LOC100511240 Protein, Phpt1 Protein
    Background
    PHPT1 is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides. Recombinant human PHPT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
    Molecular Weight
    15.9 kDa (145aa), confirmed by MALDI-TOF
    NCBI Accession
    NP_054891
    UniProt
    Q9NRX4
    Pathways
    Positive Regulation of Peptide Hormone Secretion
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