PKM Protein (AA 1-531) (His tag)

Catalog No. ABIN6388051

Product Details

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 1-531

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PKM protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSH MGSMPKPHSE AGTAFIQTQQ LHAAMADTFL EHMCRLDIDS APITARNTGI ICTIGPASRS VEMLKEMIKS GMNVARLNFS HGTHEYHAET IKNVREATES FASDPILYRP VAVALDTKGP EIRTGLIKGS GTAEVELKKG ATLKITLDNA YMEKCDENIL WLDYKNICKV VEVGSKIYVD DGLISLQVKE KGADFLVTEV ENGGSLGSKK GVNLPGAAVD LPAVSEKDIQ DLKFGVEQDV DMVFASFIRK AADVHEVRKV LGEKGKNIKI ISKIENHEGV RRFDEILEAS DGIMVARGDL GIEIPAEKVF LAQKMMIGRC NRAGKPVICA TQMLESMIKK PRPTRAEGSD VANAVLDGAD CIMLSGETAK GDYPLEAVRM QHLIAREAEA AIYHLQLFEE LRRLAPITSD PTEAAAVGAV EASFKCCSGA IIVLTKSGRS AHQVARYRPR APIIAVTRNP QTARQAHLYR GIFPVLCKDA VLNAWAEDVD LRVNLAMDVG KARGFFKKGD VVIVLTGWRP GSGFTNTMRV VPVP

Purity: > 85 % by SDS - PAGE

Biological Activity Comment: Specific activity: > 50,000 pmol/min/ug. One unit will convert 1.0 pmole of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37C

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Bioactivity Validated

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl(  pH 8.5) containing 0.2M NaCl, 1  mM DTT, 30 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Alternative Name: Pkm (PKM Products)

Synonyms: CTHBP Protein, OIP3 Protein, PK3 Protein, PKM2 Protein, TCB Protein, THBP1 Protein, AA414905 Protein, AL024370 Protein, AL024424 Protein, Pk-2 Protein, Pk-3 Protein, Pk3 Protein, Pkm2 Protein, PKM12 Protein, CG7070 Protein, Dmel\\CG7070 Protein, PYK Protein, Pyk Protein, cb1029 Protein, fj33f03 Protein, pkm2 Protein, pkm2a Protein, wu:fj33f03 Protein, MGC53388 Protein, PKM Protein, PK-S Protein, pk3 Protein, pkm2b Protein, zgc:92037 Protein, pyruvate kinase M1/2 Protein, pyruvate kinase, muscle Protein, Pyruvate kinase Protein, pyruvate kinase M1/2a Protein, pyruvate kinase PKM Protein, pyruvate kinase (PK) Protein, pyruvate kinase Protein, pyruvate kinase M1/2 S homeolog Protein, pyruvate kinase M1/2b Protein, PKM Protein, Pkm Protein, PyK Protein, pkma Protein, pkm Protein, LOC710798 Protein, pyk Protein, pk Protein, LOC100534543 Protein, pkm.S Protein, pkmb Protein

Background: Pkm, also known as pyruvate kinase PKM isoform M2, is an isoenzyme of the glycolytic enzyme pyruvate kinase. This protein catalyzes the production of pyruvate and ATP from phosphoenolpyruvate. Pkm interacting with Opa proteins, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, is required for bacterial pathogenesis. It is specifically expressed at high levels in tumor cells, and can be measured in plasma of patients with advanced breast cancer. Recombinant mouse Pkm, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 60.2 kDa (554aa) Confirmed by MALDI-TOF

NCBI Accession: NP_035229

UniProt: P52480

Pathways: Warburg Effect