NNMT Protein (AA 1-264) (His tag)
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- Target See all NNMT Proteins
- NNMT (Nicotinamide N-Methyltransferase (NNMT))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-264
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NNMT protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MESGFTSKDT YLSHFNPRDY LEKYYKFGSR HSAESQILKH LLKNLFKIFC LDGVKGDLLI DIGSGPTIYQ LLSACESFKE IVVTDYSDQN LQELEKWLKK EPEAFDWSPV VTYVCDLEGN RVKGPEKEEK LRQAVKQVLK CDVTQSQPLG AVPLPPADCV LSTLCLDAAC PDLPTYCRAL RNLGSLLKPG GFLVIMDALK SSYYMIGEQK FSSLPLGREA VEAAVKEAGY TIEWFEVISQ SYSSTMANNE GLFSLVARKL SRPL
- Purity
- > 95 % by SDS-PAGE
- Biological Activity Comment
- Specific activity is > 100 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C.
- Top Product
- Discover our top product NNMT Protein
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Nicotinamide N-Methyltransferase (NNMT) (AA 1-264) protein (His tag)
NNMT Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Nicotinamide N-Methyltransferase (NNMT) (AA 1-264) protein (GST tag)NNMT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Nicotinamide N-Methyltransferase (NNMT) (AA 1-264) protein (Strep Tag)Crystallography grade NNMT Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Nicotinamide N-Methyltransferase (NNMT) (AA 1-264) protein (Strep Tag)Crystallography grade NNMT Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Nicotinamide N-Methyltransferase (NNMT) proteinNNMT Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, WB, ELISA, IP
Nicotinamide N-Methyltransferase (NNMT) protein (Myc-DYKDDDDK Tag)NNMT Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- NNMT (Nicotinamide N-Methyltransferase (NNMT))
- Alternative Name
- NNMT (NNMT Products)
- Synonyms
- MGC64498 Protein, Afu1g17750 Protein, AO090026000620 Protein, nnmt Protein, nicotinamide N-methyltransferase S homeolog Protein, nicotinamide N-methyltransferase Protein, nicotinamide n-methyltransferase Protein, Nicotinamide N-methyltransferase Protein, nnmt.S Protein, CNG02680 Protein, AFUA_1G17750 Protein, AOR_1_1132194 Protein, AOR_1_1124014 Protein, CC1G_02598 Protein, VDBG_08111 Protein, TERG_00572 Protein, Tsp_04293 Protein, nnmt Protein, NNMT Protein, Nnmt Protein
- Background
- NNMT(Nicotinamide N-methyltransferase) belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. It is predominantly expressed in the liver, and a lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. NNMT catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. Recombinant human NNMT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
- Molecular Weight
- 37.7 kDa (284aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_006160
- UniProt
- P40261
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